Ochiai A, Akimoto S, Shimoyama Y, Nagafuchi A, Tsukita S, Hirohashi S
Pathology Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1994 Mar;85(3):266-73. doi: 10.1111/j.1349-7006.1994.tb02092.x.
To investigate the mechanisms of disruption of cell-cell contact in scirrhous carcinoma cells, the expression of both E-cadherin and alpha catenin, which is an intracellular cadherin-binding molecule, were determined in scirrhous-type adenocarcinomas of the stomach and breast using immunohistochemical and immunoblotting techniques. The losses of E-cadherin expression in gastric and breast scirrhous adenocarcinomas were 18.1% and 0%, respectively, and those of alpha catenin expression were 54.6% and 75%, respectively. Frequent loss of alpha catenin expression occurred in scirrhous carcinomas with scattered cell growth in the stomach and the breast and showed no organ specificity. In addition, all the infiltrating lobular carcinomas, which also infiltrate the stroma as single cells, showed no E-cadherin or alpha catenin expression. These findings suggest that down-regulation of either alpha catenin or E-cadherin plays a critical role in the disruption of cell adhesion in carcinomas with scattered cell growth.
为了研究硬癌癌细胞中细胞间接触破坏的机制，我们使用免疫组织化学和免疫印迹技术，检测了E-钙黏蛋白和α-连环蛋白（一种细胞内钙黏蛋白结合分子）在胃和乳腺硬癌型腺癌中的表达。胃和乳腺硬癌腺癌中E-钙黏蛋白表达缺失率分别为18.1%和0%，α-连环蛋白表达缺失率分别为54.6%和75%。α-连环蛋白表达缺失在胃和乳腺中散在细胞生长的硬癌中频繁发生，且无器官特异性。此外，所有同样以单个细胞浸润基质的浸润性小叶癌均未显示E-钙黏蛋白或α-连环蛋白表达。这些发现表明，α-连环蛋白或E-钙黏蛋白的下调在散在细胞生长的癌中细胞黏附破坏中起关键作用。
