Ruiz-Gutierrez V, Vazquez C M, Quintero F J
Instituto de la Grasa y sus Derivados, CSIC, Sevilla, España.
J Biochem. 1994 Jan;115(1):32-6. doi: 10.1093/oxfordjournals.jbchem.a124300.
In this study we examined the effect of 50% distal small bowel resection (DSBR) on the lipid composition of rat colonic mucosa, including the fatty acid composition of major phospholipid classes, phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), lysophosphatidylcholine (LPC), and sphingomyelin (SM). Phospholipid, free cholesterol, triacylglycerols, diacylglycerols, and cholesterol ester levels were not affected by the surgical operation. PE together with PC accounted for more than 60% of total phospholipid, in both groups of animals. The relative percentages of PE, PC, PI, SM, and cardiolipin (CL) remained unaffected by DSBR. However, a decrease in the content of LPC was observed in 50%-resected animals. Different fatty acid composition changes in the colonic phospholipid classes were observed after resection. PC fraction contained the highest amounts of saturated fatty acids, but monounsaturated fatty acids were present in high levels in PI fraction, in both groups of animals. DSBR produces different changes in the levels of linoleic and arachidonic acids. These results suggest that the lipid composition and the fatty acid profile of the different phospholipid fractions change in the adaptive response of colon to intestinal resection.
在本研究中，我们检测了50%远端小肠切除术（DSBR）对大鼠结肠黏膜脂质组成的影响，包括主要磷脂类别的脂肪酸组成，即磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）、磷脂酰肌醇（PI）、溶血磷脂酰胆碱（LPC）和鞘磷脂（SM）。磷脂、游离胆固醇、三酰甘油、二酰甘油和胆固醇酯水平不受手术操作的影响。在两组动物中，PE和PC一起占总磷脂的60%以上。PE、PC、PI、SM和心磷脂（CL）的相对百分比不受DSBR的影响。然而，在50%切除的动物中观察到LPC含量降低。切除术后，在结肠磷脂类中观察到不同的脂肪酸组成变化。在两组动物中，PC组分含有最高量的饱和脂肪酸，但PI组分中含有高水平的单不饱和脂肪酸。DSBR使亚油酸和花生四烯酸水平产生不同变化。这些结果表明，在结肠对肠切除的适应性反应中，不同磷脂组分的脂质组成和脂肪酸谱发生了变化。
