Hrouda M, Paszkowski J
Research Institute for Corp Production, Praha, Czech Republic.
Mol Gen Genet. 1994 Apr;243(1):106-11. doi: 10.1007/BF00283882.
The precision of extrachromosomal homologous recombination and gene targeting in plant cells was investigated. Recombination was directed to introns of selectable marker genes where potential changes could persist without affecting the function and therefore the selectability of the genes. Approximately 9 kb of crossover regions was rescued and sequenced. Changes were detected at a frequency below one point mutation per 1000 bp, indicating that extrachromosomal recombination and gene targeting both appear to occur with high fidelity.
对植物细胞中染色体外同源重组和基因靶向的精确性进行了研究。重组被导向可选择标记基因的内含子，在这些位置潜在的变化可以持续存在而不影响基因的功能，进而不影响其可选择特性。大约9kb的交叉区域被挽救并测序。检测到的变化频率低于每1000bp一个点突变，这表明染色体外重组和基因靶向似乎都以高保真度发生。
