INRA Station d'amélioration des plantes, BP 29, 35650, Le Rheu, France.
Theor Appl Genet. 1995 Nov;91(6-7):956-63. doi: 10.1007/BF00223906.
Spontaneous outcrossing of different malesterile rapeseed lines and transgenic hybrids with a population of a weedy species, Raphanus raphanistrum L., has led to the harvest of numerous seeds showing a size dimorphism. Flow cytometry analysis correlated with chromosome counts showed that all of the large seeds belonged to rapeseed, whereas the small seeds were a mixture of mostly interspecific triploid hybrids, with some trigenomic amphidiploids, diploid and haploid rapeseed plants. Significant differences were revealed between the rapeseed lines and transgenic hybrids in their ability to form interspecific hybrids with Raphanus raphanistrum under natural conditions. Resistance to the herbicide Basta was properly expressed in the triploid and amphidiploid hybrids. Low male fertility of the interspecific triploid hybrids was not correlated with seed set in the subsequent generation.
不同不育油菜品系和与野生种萝卜（Raphanus raphanistrum L.）杂交的转基因杂种与该野生种的群体自然杂交，产生了大量表现出大小二型的种子。流式细胞术分析与染色体计数相关联的结果表明，所有大种子都属于油菜，而小种子则是主要的种间三倍体杂种的混合物，还有一些三基因组的双二倍体、二倍体和一倍体油菜植株。在自然条件下，油菜品系和转基因杂种与萝卜形成种间杂种的能力存在显著差异。三倍体和双二倍体杂种对除草剂 Basta 表现出适当的抗性。种间三倍体杂种的雄性育性低与后续代的种子结实率没有相关性。
