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塑料板中病毒感染细胞培养物的荧光抗体染色程序。

Procedure for fluorescent-antibody staining of virus-infected cell cultures in plastic plates.

作者信息

Pursell A R, Cole J R

出版信息

J Clin Microbiol. 1976 May;3(5):537-40. doi: 10.1128/jcm.3.5.537-540.1976.

DOI:10.1128/jcm.3.5.537-540.1976
PMID:819456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274345/
Abstract

Acetone fixation and fluorescent-antibody staining of virus-infected cell cultures were performed in plastic plates. Proper addition of acetone as a fixative did not alter the plastic.

摘要

在塑料培养板中对病毒感染的细胞培养物进行丙酮固定和荧光抗体染色。适当添加丙酮作为固定剂不会改变塑料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/9c30a073f3df/jcm00226-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/a49ec9baf037/jcm00226-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/7d6b751e181f/jcm00226-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/59e7e16b1474/jcm00226-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/9c30a073f3df/jcm00226-0103-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/a49ec9baf037/jcm00226-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/7d6b751e181f/jcm00226-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/59e7e16b1474/jcm00226-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b767/274345/9c30a073f3df/jcm00226-0103-b.jpg

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本文引用的文献

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5
Porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigs.猪副轮状病毒：在无菌猪中的检测、与轮状病毒的鉴别及发病机制
J Clin Microbiol. 1982 Feb;15(2):312-9. doi: 10.1128/jcm.15.2.312-319.1982.
6
Microneutralization test for respiratory syncytial virus based on an enzyme immunoassay.基于酶免疫测定的呼吸道合胞病毒微中和试验
J Clin Microbiol. 1985 Dec;22(6):1050-2. doi: 10.1128/jcm.22.6.1050-1052.1985.
7
Application of a modified indirect fluorescent antibody test to the detection of antibodies to bovine respiratory syncytial virus in Ontario cattle.一种改良的间接荧光抗体试验在安大略省牛群中检测牛呼吸道合胞体病毒抗体的应用。
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J Clin Microbiol. 1977 Mar;5(3):361-4. doi: 10.1128/jcm.5.3.361-364.1977.