基于酶免疫测定的呼吸道合胞病毒微中和试验
Microneutralization test for respiratory syncytial virus based on an enzyme immunoassay.
作者信息
Anderson L J, Hierholzer J C, Bingham P G, Stone Y O
出版信息
J Clin Microbiol. 1985 Dec;22(6):1050-2. doi: 10.1128/jcm.22.6.1050-1052.1985.
Abstract
Virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. After an appropriate incubation period, the cells were fixed, and an enzyme-linked immunosorbent assay was performed directly in the microtiter plates for detection of virus. Results could be read and recorded automatically, which is especially helpful when running large numbers of tests.
摘要
在微量滴定板的细胞培养物中测定呼吸道合胞病毒的病毒感染力和抗体中和滴度。经过适当的孵育期后，将细胞固定，并直接在微量滴定板中进行酶联免疫吸附测定以检测病毒。结果可以自动读取和记录，这在进行大量测试时特别有用。
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