Ohman J L, Bloch K J, Kendall S, Lowell F C
J Allergy Clin Immunol. 1976 Jun;57(6):560-8. doi: 10.1016/0091-6749(76)90007-5.
IgE antibodies present in serum from 3 patients clinically sensitive to cat and dog were shown to combine with whole cat and dog sera linked to cellulose particles. Employing a modified radioallergosorbent technique (RAST), it was shown that cat and dog sera inhibited the binding of IgE antibodies to insolubilized cat serum and to insolubilized dog serum. These findings suggest that cat and dog sera have common allergens. Other mammalian sera were also tested and found to have little inhibitory activity. Cat and dog sera did not inhibit the binding of IgE antiragweed antibodies to an insolubilized ragweed fraction. Following separation of cat serum by gel filtration on Sephadex G-200 or electrophoresis in acrylamide gel, several fractions were found to inhibit the binding of IgE antibodies to insolubilized cat and dog sera. Whether the heterogeneity observed reflects the presence of single allergens with diverse size and charge properties or multiple allergens remains to be determined.
研究表明，来自3名对猫和狗临床敏感患者血清中的IgE抗体，可与结合在纤维素颗粒上的全猫血清和全狗血清相结合。采用改良的放射变应原吸附技术（RAST）发现，猫血清和狗血清可抑制IgE抗体与固定化猫血清及固定化狗血清的结合。这些发现提示猫血清和狗血清存在共同变应原。还对其他哺乳动物血清进行了检测，发现其抑制活性很小。猫血清和狗血清不能抑制IgE抗豚草抗体与固定化豚草组分的结合。通过Sephadex G - 200凝胶过滤或丙烯酰胺凝胶电泳分离猫血清后，发现有几个组分可抑制IgE抗体与固定化猫血清和狗血清的结合。观察到的异质性是反映了具有不同大小和电荷性质的单一变应原的存在，还是多种变应原的存在，仍有待确定。
