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分离感染植物病原菌的新型噬菌体。

Isolation of Novel Phages Infecting the Plant Pathogens and .

机构信息

Institute of Bio- and Geosciences, Department for Plant Sciences (IBG-2), Forschungszentrum Jülich, 52425 Jülich, Germany.

Institute of Bio- and Geosciences, Department for Biotechnology (IBG-1), Forschungszentrum Jülich, 52425 Jülich, Germany.

出版信息

Viruses. 2022 Jun 30;14(7):1449. doi: 10.3390/v14071449.

DOI:10.3390/v14071449
PMID:35891434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9316219/
Abstract

The genus of contains many well-known plant pathogens with the ability to infect some of the most important crop plants, thereby causing significant economic damage. Unfortunately, classical pest-control strategies are neither particularly efficient nor sustainable and we are, therefore, in demand of alternatives. Here, we present the isolation and characterization of seven novel phages infecting the plant-pathogenic species and . Transmission electron microscopy revealed that all phages show a siphovirion morphology. The analysis of genome sequences and plaque morphologies are in agreement with a lytic lifestyle of the phages making them suitable candidates for biocontrol. Moreover, three of the isolated phages form the new genus "". All seven phages belong to four distinct clusters underpinning their phylogenetic diversity. Altogether, this study presents the first characterized isolates for the plant pathogen and expands the number of available phages for plant biocontrol.

摘要

包含许多著名的植物病原体,这些病原体能够感染一些最重要的农作物,从而造成重大的经济损失。不幸的是,传统的害虫防治策略既不特别有效也不具有可持续性,因此我们需要替代品。在这里,我们介绍了七种新型噬菌体的分离和特性,这些噬菌体感染植物病原物种和。透射电子显微镜显示,所有噬菌体均表现出长尾噬菌体的形态。基因组序列和蚀斑形态的分析表明,噬菌体具有裂解生活方式,使其成为生物防治的合适候选者。此外,分离出的三种噬菌体形成了新的属“”。七种噬菌体属于四个不同的聚类,这支持了它们的系统发育多样性。总的来说,这项研究首次对植物病原体进行了特征描述,并扩大了可用于植物生物防治的噬菌体数量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/199eb3ae59ea/viruses-14-01449-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/cb988e76d699/viruses-14-01449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/39ef603809a3/viruses-14-01449-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/b9e92f4173a3/viruses-14-01449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/8a4c987f07fc/viruses-14-01449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/199eb3ae59ea/viruses-14-01449-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/cb988e76d699/viruses-14-01449-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/39ef603809a3/viruses-14-01449-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/b9e92f4173a3/viruses-14-01449-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/8a4c987f07fc/viruses-14-01449-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf87/9316219/199eb3ae59ea/viruses-14-01449-g005.jpg

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