Williams R J
J Med Microbiol. 1976 May;9(2):153-61. doi: 10.1099/00222615-9-2-153.
The interactions of a contractile, a filamentous and a small pyocine with a sensitive strain of Pseudomonas aeruginosa (no. P14) were examined in vivo. The purification procedure used yielded high-activity pyocine preparations that were not toxic to mice. The inhibitory activity of such preparations, when injected into mice by various routes, was retained for up to 24 h. However, high molecular-weight pyocines given intraperitoneally in the presence of a lethal dose of strain P14 administered by the same route did not prevent the fatal outcome of infection unless they are given before or together with the bacteria. The small pyocine had no protective effect. In burned mice infected with strain P14, topical application of a filamentous pyocine failed to improve the chances of survival. The results suggest that there is little future for pyocine therapy.
在体内研究了一种收缩型、一种丝状型和一种小分子绿脓菌素与铜绿假单胞菌敏感菌株（编号P14）的相互作用。所采用的纯化程序得到了对小鼠无毒的高活性绿脓菌素制剂。通过各种途径注射到小鼠体内时，此类制剂的抑制活性可保持长达24小时。然而，在经相同途径给予致死剂量的P14菌株的情况下，腹腔内给予高分子量绿脓菌素并不能防止感染导致的致命结局，除非在细菌之前或与细菌同时给予。小分子绿脓菌素没有保护作用。在感染P14菌株的烧伤小鼠中，局部应用丝状绿脓菌素未能提高存活几率。结果表明绿脓菌素疗法前景渺茫。
