Li L, Elledge S J, Peterson C A, Bales E S, Legerski R J
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Proc Natl Acad Sci U S A. 1994 May 24;91(11):5012-6. doi: 10.1073/pnas.91.11.5012.
Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.
真核细胞中核苷酸切除修复途径对DNA损伤的处理很可能是由多蛋白复合物完成的。然而，这些复合物的性质以及DNA修复因子之间分子相互作用的细节在很大程度上尚不清楚。在这里，我们通过双杂交系统在体内以及使用重组蛋白在体外证明，人类修复因子XPA和ERCC1特异性相互作用。此外，我们报告了对ERCC1和XPA中介导这种相互作用的结构域的初步确定。这些结果表明，XPA可能在由ERCC1和可能的其他修复因子组成的切口复合物定位或加载到损伤位点的过程中发挥作用。
