Goudswaard J, Gilmour N J, Dijkstra R G, van Beek J J
Vet Rec. 1976 Jun 5;98(23):461-2. doi: 10.1136/vr.98.23.461.
Sera from 775 adult milking cattle on 13 farms in Friesland and from 359 of the same cattle taken during the following year, were examined for evidence of infection with M johnei by the complement fixation, fluorescent antibody, haemagglutination, haemagglutination-lysis and immunodiffusion tests. Eighteen animals, not clinically affected but which had serologically positive tests were slaughtered and M johnei was isolated in cultures from 16. The fluorescent antibody test was as sensitive as the complement fixation test. The other tests were relatively insensitive and would only be of value as confirmatory tests in the presence of reactions to the first two tests. It is concluded that the use of the fluorescent antibody and the complement fixation test together was of value in detecting sub-clinical Mycobacterium johnei infection.
对弗里斯兰省13个农场的775头成年泌乳奶牛的血清，以及次年采集的其中359头同一批奶牛的血清，通过补体结合试验、荧光抗体试验、血凝试验、血凝溶解试验和免疫扩散试验，检测是否感染副结核分枝杆菌。对18头无临床症状但血清学检测呈阳性的动物进行屠宰，从16头动物的培养物中分离出副结核分枝杆菌。荧光抗体试验与补体结合试验的敏感性相当。其他试验相对不敏感，仅在前两项试验呈阳性反应时作为确证试验才有价值。得出结论，联合使用荧光抗体试验和补体结合试验对检测亚临床副结核分枝杆菌感染有价值。
