人乳头瘤病毒16型E6基因转录本的致瘤性与表达水平或剪接模式之间的相关性
Correlation between tumorigenicity and expression levels or splicing patterns of transcripts of the human papillomavirus type 16 E6 gene.
作者信息
Inoue T, Kyo S, Kiyono T, Ishibashi M, Ishiwatari H, Hwang Y I, Yutsudo M, Hakura A
机构信息
Department of Tumor Virology, Osaka University.
出版信息
Jpn J Cancer Res. 1994 Apr;85(4):357-63. doi: 10.1111/j.1349-7006.1994.tb02367.x.
Abstract
For determination of the correlation between tumorigenicity and the expression levels or splicing patterns of E6 mRNAs of the human papillomavirus type 16 in established cells, a vector containing the intact E6 open reading frame which expresses both unspliced and spliced mRNAs, one expressing only unspliced E6 mRNA, and one expressing both unspliced and spliced mRNAs but producing only truncated E6 proteins were constructed. In transformation assays and analyses of E6 mRNAs, a higher expression level of unspliced E6 mRNA was found to be closely associated with tumorigenicity. Furthermore, it was also related with anchorage-independent growth and a decreased serum requirement of the cells.
摘要
为了确定在已建立的细胞中，人乳头瘤病毒16型E6 mRNA的致瘤性与表达水平或剪接模式之间的相关性，构建了三种载体：一种含有完整E6开放阅读框，可同时表达未剪接和剪接的mRNA；一种仅表达未剪接的E6 mRNA；还有一种表达未剪接和剪接的mRNA，但仅产生截短的E6蛋白。在转化试验和E6 mRNA分析中，发现未剪接的E6 mRNA较高的表达水平与致瘤性密切相关。此外，它还与细胞的不依赖贴壁生长及血清需求降低有关。
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