大肠杆菌基因组的系统测序：2.4 - 4.1分钟（110,917 - 193,643碱基对）区域的分析
Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region.
作者信息
Fujita N, Mori H, Yura T, Ishihama A
机构信息
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
出版信息
Nucleic Acids Res. 1994 May 11;22(9):1637-9. doi: 10.1093/nar/22.9.1637.
Abstract
The complete sequence analysis of the E. coli genome was initiated as a collaborative study in Japan. Following the initial analysis of the 0-2.4 min region (Yura, T. et al. (1992) Nucleic Acids Res. 20, 3305-3308), a contiguous sequence of 82,727 bp corresponding to the 2.4-4.1 min region (110,917-193,643 bp as counted from 0 min) was determined. The resulting sequence was found to contain at least 33 known genes and 24 putative genes predicted from protein sequence homology.
摘要
大肠杆菌基因组的全序列分析是在日本开展的一项合作研究。在对0 - 2.4分钟区域进行初步分析之后（Yura, T.等人，(1992)《核酸研究》20, 3305 - 3308），确定了对应于2.4 - 4.1分钟区域（从0分钟起计为110,917 - 193,643碱基对）的82,727碱基对的连续序列。结果发现所得序列至少包含33个已知基因以及根据蛋白质序列同源性预测的24个推定基因。
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