通过荧光激活细胞分选获得的双芽巴贝斯虫克隆显示出核型多态性。
Fluorescence-activated cell sorting-derived clones of Babesia bigemina show karyotype polymorphism.
作者信息
Estes D M, Bailey C W, Barnett L, Lafrenz D, Brandt H M, Jensen J B, Allen G K, Carson C A
机构信息
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
出版信息
Parasitol Res. 1994;80(2):104-7. doi: 10.1007/BF00933775.
PMID:8202447
Abstract
Use of the fluorescence-activated cell sorter proved to be an accurate and highly efficient means for cloning Babesia parasites. These qualities were examined by separating a mixed population of Babesia-infected bovine erythrocytes composed of two isolates with different karyotypes. Direct evidence of polymorphism was detected during comparison of the resultant clones.
摘要
事实证明，使用荧光激活细胞分选仪是克隆巴贝斯虫寄生虫的一种准确且高效的方法。通过分离由两种具有不同核型的分离株组成的感染巴贝斯虫的牛红细胞混合群体来检验这些特性。在对所得克隆进行比较的过程中检测到了多态性的直接证据。
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