空气中颗粒物对人巨噬细胞吞噬作用的抑制

Inhibition of phagocytosis of human macrophages induced by airborne particulates.

作者信息

Hadnagy W, Seemayer N H

机构信息

Institute of Hygiene, Heinrich-Heine-University Düsseldorf, Germany.

出版信息

Toxicol Lett. 1994 Jun;72(1-3):23-31. doi: 10.1016/0378-4274(94)90006-x.

DOI:10.1016/0378-4274(94)90006-x

PMID:8202937

Abstract

Human monocyte-derived macrophages isolated from peripheral blood were treated with different extracts of airborne particulates collected in the highly industrialized Rhine-Ruhr area. All tested extracts showed a substantial impairment of phagocytosis by inhibition of phagocytic activity as well as phagocytic capacity, while cell viability was rather well maintained. Significant reduction of phagocytosis already appeared at a concentration equivalent to extracted particulates from 3.8 m3 air. Having properties of alveolar macrophages, human monocyte-derived macrophage cultures may offer a reliable in vitro model for assessment of pulmonary toxicity by respirable pollutants.

摘要

从外周血中分离出的人单核细胞衍生巨噬细胞，用在高度工业化的莱茵 - 鲁尔地区收集的空气颗粒物的不同提取物进行处理。所有测试提取物均通过抑制吞噬活性以及吞噬能力，显示出吞噬作用的显著损害，而细胞活力则得到较好维持。在相当于从3.8立方米空气中提取的颗粒物浓度时，吞噬作用就已出现显著降低。具有肺泡巨噬细胞特性的人单核细胞衍生巨噬细胞培养物，可能为评估可吸入污染物的肺毒性提供一个可靠的体外模型。