Amella C A, Lofgren L A, Ronn A M, Nouri M, Shikowitz M J, Steinberg B M
Department of Otolaryngology and Communicative Disorders, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Am J Pathol. 1994 Jun;144(6):1167-71.
Latent human papillomavirus infection, a very common event, is most likely the source of primary and recurrent papillomas of the respiratory and genital tracts and might also be the source of neoplastic lesions of the female genital tract and the penis. We have developed a simple model for papillomavirus latency using cottontail rabbit papillomavirus. Skin of domestic rabbits was minimally scarified and inoculated with dilutions of a crude virus suspension ranging from 200 ng to 20 pg viral DNA per inoculated site. Dilution of virus to less than 10 ng/site resulted in delayed and reduced efficiency of inducing warts. After follow-up of 1 to 6 months, sites immediately adjacent to papillomas and inoculated sites where papillomas did not form were biopsied and analyzed by Southern blot and polymerase chain reaction. Inoculated tissues that were clinically and histologically normal contained viral DNA at low levels, detectable by polymerase chain reaction. Ability of the latent virus to induce warts was confirmed by activation with mild skin irritation causing wart formation. This simple model system for latent papillomavirus can be used to study mechanisms of viral activation, therapies to prevent activation, and therapies to eliminate latent virus and thus cure the infection.
人乳头瘤病毒潜伏感染是一种非常常见的现象，很可能是呼吸道和生殖道原发性及复发性乳头瘤的来源，也可能是女性生殖道和阴茎肿瘤性病变的来源。我们利用棉尾兔乳头瘤病毒开发了一种简单的乳头瘤病毒潜伏模型。对家兔皮肤进行轻微划痕处理，然后在每个接种部位接种稀释度从200纳克到20皮克病毒DNA的粗病毒悬液。将病毒稀释至低于10纳克/部位会导致诱导疣的效率延迟且降低。在随访1至6个月后，对紧邻乳头瘤的部位以及未形成乳头瘤的接种部位进行活检，并通过Southern印迹法和聚合酶链反应进行分析。临床上和组织学上正常的接种组织含有低水平的病毒DNA，可通过聚合酶链反应检测到。通过轻度皮肤刺激激活潜伏病毒导致疣形成，从而证实了潜伏病毒诱导疣的能力。这个简单的乳头瘤病毒潜伏模型系统可用于研究病毒激活机制、预防激活的疗法以及消除潜伏病毒从而治愈感染的疗法。
