Sasaki O, Soejima K
Dept. of Surgery, Fukuoka Dental College.
Gan To Kagaku Ryoho. 1994 May;21 Suppl 1:27-31.
Flow cytometric determination of DNA ploidy was performed on paraffin-embedded specimens of primary tumor (Group A) and its metastatic lymph nodes (Group B) in 30 surgically resected cases, and the DNA index (DI) was compared between these two groups. In 25 of 30 cases (83.3%), all DI observed in Group B were also found in Group A; and in the remaining 5 cases, a series of DI not found in Group A were observed in group B. They were all poorly differentiated adenocarcinomas, 4 of which exhibited serosal invasion and prominent lymphatic permeation in their wall. In 28 cases (93.3%), the modal DI value in the primary tumor was also found in their corresponding metastatic lymph node, the frequency of which revealed a statistically significant difference from 18 cases (60%) in terms of median DI value, and from 10 cases (33.3%) in terms of maximum DI value (p < 0.01). It was suggested that the modal DI value in the primary tumor could signify an important clone relating to lymph node metastasis. No significant DI difference was observed in comparison of a series of DI seen in n1(+) and n2(+) group of metastatic regional lymph nodes.
对30例手术切除病例的原发性肿瘤石蜡包埋标本（A组）及其转移淋巴结（B组）进行流式细胞术DNA倍体测定，并比较两组之间的DNA指数（DI）。30例中的25例（83.3%），B组观察到的所有DI在A组中也有发现；其余5例中，B组观察到一系列A组未发现的DI。它们均为低分化腺癌，其中4例在其壁内表现出浆膜侵犯和明显的淋巴管浸润。28例（93.3%）中，原发性肿瘤的众数DI值在其相应的转移淋巴结中也有发现，其频率在中位数DI值方面与18例（60%）有统计学显著差异，在最大DI值方面与10例（33.3%）有统计学显著差异（p<0.01）。提示原发性肿瘤的众数DI值可能代表与淋巴结转移相关的一个重要克隆。在比较转移区域淋巴结的n1(+)和n2(+)组中观察到的一系列DI时，未观察到显著的DI差异。
