Mimori K, Mori M, Matsushima T, Adachi Y, Kuwano H, Sugimachi K
Dept. of Surgery II, Kyushu University School of Medicine.
Gan To Kagaku Ryoho. 1994 May;21 Suppl 1:37-40.
We performed DNA flow cytometry analysis of colon carcinomas from the viewpoint of DNA heterogeneity. The materials were 25 colon carcinomas and 17 metastatic lymph nodes. Four different regions were examined in each primary tumor. The DNA index (DI) was classified into four groups. We classified the primary tumors into homogeneous and heterogeneous group according to the DI of the four regions of each case. Ten of 25 (40%) were classified as homogeneous and 15 (60%) as heterogeneous. The heterogeneous group tended to show more aggressive clinicopathologic characteristics. The corresponding rate between the maximum DI in the primary tumor and the DI in the metastatic lymph node was 65% (11/17). The maximum DI of the primary tumors was mostly seen in the metastatic lymph nodes, suggesting high metastatic potentiality of the higher DI clones. The examination of DNA heterogeneity may be useful for detecting the more precise character of the colon carcinomas. In heterogeneous cases, the higher corresponding rate of DI between primary and metastatic lesions suggested that metastasis occurred frequently in primary lesions containing gathering larger DI cells.
我们从DNA异质性的角度对结肠癌进行了DNA流式细胞术分析。材料包括25例结肠癌和17个转移淋巴结。对每个原发性肿瘤检查四个不同区域。DNA指数（DI）分为四组。我们根据每个病例四个区域的DI将原发性肿瘤分为同质性和异质性组。25例中有10例（40%）被分类为同质性，15例（60%）为异质性。异质性组倾向于表现出更具侵袭性的临床病理特征。原发性肿瘤的最大DI与转移淋巴结中的DI之间的对应率为65%（11/17）。原发性肿瘤的最大DI大多出现在转移淋巴结中，表明较高DI克隆具有较高的转移潜能。DNA异质性检查可能有助于检测结肠癌更精确的特征。在异质性病例中，原发性和转移性病变之间较高的DI对应率表明，转移在含有聚集较大DI细胞的原发性病变中频繁发生。
