Singh R K, Gutman M, Radinsky R, Bucana C D, Fidler I J
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Cancer Res. 1994 Jun 15;54(12):3242-7.
We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.
我们将13种不同的人黑色素瘤细胞系中白细胞介素8（IL-8）的稳态转录和蛋白分泌与其在裸鼠体内生长和产生转移的能力进行了关联。高转移性细胞比低转移性细胞表达更高水平的IL-8 mRNA转录本稳态。原位mRNA杂交分析在细胞水平上证实了mRNA表达模式。如通过酶联免疫吸附测定所确定的，mRNA表达增加与IL-8蛋白分泌直接相关。重组IL-8以剂量依赖性方式刺激低转移性A375P细胞的增殖，使用抗IL-8多克隆抗体可消除这种刺激。数据表明IL-8可能是人黑色素瘤细胞的自分泌生长因子，并且IL-8参与黑色素瘤转移。
