影响男性沙眼衣原体检测中尿液酶免疫分析敏感性的因素。
Factors affecting urine EIA sensitivity in the detection of Chlamydia trachomatis in men.
作者信息
Talbot H, Romanowski B
机构信息
Clinical Investigation Unit, STD Services, Alberta Health, Edmonton, Canada.
出版信息
Genitourin Med. 1994 Apr;70(2):101-4. doi: 10.1136/sti.70.2.101.
OBJECTIVE
This study examined the effects of four variables on the detection of Chlamydia trachomatis in urine from men by enzyme immunoassay (EIA). These variables were: symptoms and signs of urethritis, urine polymorphonuclear leucocytes (PMN), inclusion counts from urethral chlamydia cell cultures and the time between testing and last voiding.
METHODS
Included were patients with and without symptoms and/or signs of urethritis attending the Edmonton Sexually Transmitted Disease Clinic. Men were asked to submit a 20 ml volume urine sample. Urethral swabs were collected for gram stain, chlamydia and gonorrhea culture.
RESULTS
A total of 318 men were evaluated of whom 47 had chlamydia. Excluding six men who were coinfected with gonorrhoea, sensitivities and specificities of the Microtrak, Chlamydiazyme and IDEIA systems were 78.1% and 99.6%, 75.6% and 100%, and 80.5% and 97.8% respectively. Last void time did not affect the sensitivity. However, sensitivity was best when applied to men with severe evidence of urethritis.
CONCLUSION
There is evidence that urine EIA could be used to detect chlamydia in men with acute urethritis but not in those without signs of urethritis.
目的
本研究通过酶免疫测定法（EIA）检测了四个变量对男性尿液中沙眼衣原体检测的影响。这些变量为：尿道炎的症状和体征、尿多形核白细胞（PMN）、尿道衣原体细胞培养中的包涵体计数以及检测与上次排尿之间的时间。
方法
纳入了埃德蒙顿性传播疾病诊所出现或未出现尿道炎症状和/或体征的患者。要求男性提交20毫升尿液样本。采集尿道拭子进行革兰氏染色、衣原体和淋病培养。
结果
共评估了318名男性，其中47人感染了衣原体。排除6名同时感染淋病的男性后，Microtrak、Chlamydiazyme和IDEIA系统的敏感性和特异性分别为78.1%和99.6%、75.6%和100%、80.5%和97.8%。上次排尿时间不影响敏感性。然而，应用于有严重尿道炎证据的男性时敏感性最佳。
结论
有证据表明，尿液EIA可用于检测患有急性尿道炎的男性中的衣原体，但不适用于无尿道炎体征的男性。
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