用于检测沙眼衣原体尿液样本的程序简化。
Simplification of procedures used to test urine samples for Chlamydia trachomatis.
作者信息
Thomas B J, Gilchrist C, Hay P E, Taylor-Robinson D
机构信息
Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex.
出版信息
J Clin Pathol. 1991 May;44(5):374-5. doi: 10.1136/jcp.44.5.374.
Abstract
Two methods of simplifying the procedure for examining urine samples for Chlamydia trachomatis were investigated. When 73 urine samples from 56 men with acute non-gonococcal urethritis were examined by direct immunofluorescence (MicroTrak), centrifuging 1 ml volumes of urine at 13,000 rpm for five minutes was at least as efficient for detecting C trachomatis as centrifuging larger volumes at 3000 rpm for 30 minutes. Furthermore, examination of urine produced during a visit to a sexually transmitted disease clinic was at least as efficient as examination of early morning urine for detecting C trachomatis by MicroTrak, or by an enzyme immunoassay (IDEIA). Both modifications have practical advantages and should encourage the use of urine samples for diagnosing chlamydial infections in men.
摘要
研究了两种简化沙眼衣原体尿液样本检测程序的方法。当采用直接免疫荧光法（MicroTrak）对来自56名急性非淋菌性尿道炎男性的73份尿液样本进行检测时，以13,000转/分钟的速度将1毫升尿液离心5分钟，在检测沙眼衣原体方面至少与以3000转/分钟的速度将更大体积的尿液离心30分钟一样有效。此外，在性传播疾病诊所就诊时采集的尿液检测沙眼衣原体，其效率至少与清晨尿液检测相当，无论是通过MicroTrak还是酶免疫测定法（IDEIA）。这两种改进方法都具有实际优势，应会促使人们使用尿液样本诊断男性衣原体感染。
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本文引用的文献
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Chlamydia trachomatis urethral infections in men. Prevalence, risk factors, and clinical manifestations.男性沙眼衣原体尿道炎感染。患病率、危险因素及临床表现。
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Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation.使用荧光素标记单克隆抗体检测涂片沙眼衣原体原体的敏感性：与传统衣原体分离法的比较
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