在性病门诊就诊的男性中，尿液与尿道拭子检测沙眼衣原体的比较。

Comparison of urine with urethral swabs for the detection of Chlamydia trachomatis in men attending an STD clinic.

作者信息

Ramuthaga T N, Mahomed F M, Greeff A S, Crewe-Brown H H, Vermeulen R

机构信息

Department of Microbiological Pathology, Medical University of Southern Africa, Medunsa.

出版信息

S Afr Med J. 1995 Dec;85(12):1287-9.

PMID:8600586

Abstract

Urethral swabs and first-catch urine specimens for the detection of Chlamydia trachomatis were collected from 370 black men with urethritis who attended a sexually transmitted disease (STD) clinic in Pretoria. An enzyme immunoassay (EIA) for C. trachomatis was carried out on all urethral swabs and urine specimens. Chlamydial culture and a direct immunofluorescent antibody (DFA) test (Imagen, Dako, UK) were also carried out on urethral swabs; DFA was used for confirmation of urine EIA positives. Based on culture and/or DFA, C. trachomatis was detected in 96 (26%) urethral swab specimens. The sensitivity of urine EIA investigation was 94% and the specificity 99%, compared with those of urethral swab EIA which were 97% and 99% respectively. The positive and negative predictive values for urine were 96% and 98% compared with 96% and 99% respectively for urethral swabs. Urine examination was therefore sensitive and specific compared with urethral swab examination in these STD patients. In view of this, the advantage of urine as an alternative to urethral swabs for C. trachomatis detection is that sampling is non-invasive and non-traumatic.

摘要

从比勒陀利亚一家性传播疾病（STD）诊所就诊的370名患有尿道炎的黑人男性中采集尿道拭子和首次晨尿样本，用于检测沙眼衣原体。对所有尿道拭子和尿液样本进行了沙眼衣原体酶免疫测定（EIA）。还对尿道拭子进行了衣原体培养和直接免疫荧光抗体（DFA）检测（Imagen，Dako，英国）；DFA用于确认尿液EIA阳性结果。基于培养和/或DFA检测，在96份（26%）尿道拭子样本中检测到沙眼衣原体。尿液EIA检测的灵敏度为94%，特异性为99%，而尿道拭子EIA的灵敏度和特异性分别为97%和99%。尿液检测的阳性预测值和阴性预测值分别为96%和98%，而尿道拭子检测的阳性预测值和阴性预测值分别为96%和99%。因此，在这些STD患者中，与尿道拭子检测相比，尿液检测具有较高的灵敏度和特异性。鉴于此，尿液作为替代尿道拭子检测沙眼衣原体的优势在于采样是非侵入性和无创伤性的。