使用尿液酶免疫测定法作为男性沙眼衣原体尿道炎的诊断工具。
Use of a urine enzyme immunoassay as a diagnostic tool for Chlamydia trachomatis urethritis in men.
作者信息
Schwebke J R, Clark A M, Pettinger M B, Nsubga P, Stamm W E
机构信息
Department of Medicine, University of Washington, Seattle 98195.
出版信息
J Clin Microbiol. 1991 Nov;29(11):2446-9. doi: 10.1128/jcm.29.11.2446-2449.1991.
We collected first-voided urine specimens from 659 males attending a sexually transmitted disease clinic and performed both enzyme immunoassay (EIA) for detection of chlamydial antigen and leukocyte esterase testing on these urine samples. The overall prevalence of chlamydial urethritis in the study population as determined by culture of urethral swabs was 11%. However, 46% of all men in the study had no symptoms of urethritis. Compared with urethral cultures for chlamydiae, the urine EIA had a sensitivity of 42% and a specificity of 99%. The sensitivity of the EIA strongly correlated with the amount of antigen present in culture as assessed by numbers of inclusion-forming units. The sensitivity of the leukocyte esterase test compared with that of chlamydia culture was 88%. We conclude that in this population of men, which included many patients without symptoms of urethritis, the urine EIA was a relatively insensitive means of screening for chlamydial infection.
我们收集了659名前往性传播疾病诊所就诊的男性的首次晨尿样本，并对这些尿液样本进行了检测衣原体抗原的酶免疫测定（EIA）和白细胞酯酶检测。通过尿道拭子培养确定，研究人群中衣原体尿道炎的总体患病率为11%。然而，研究中所有男性中有46%没有尿道炎症状。与衣原体尿道培养相比，尿液EIA的敏感性为42%，特异性为99%。EIA的敏感性与培养物中存在的抗原量密切相关，抗原量通过包涵体形成单位数量评估。白细胞酯酶检测与衣原体培养相比的敏感性为88%。我们得出结论，在这群男性中，包括许多没有尿道炎症状的患者，尿液EIA是一种相对不敏感的衣原体感染筛查方法。
Zotero 插件