Ferguson T A, Herndon J M
Department of Ophthalmology, Washington University School of Medicine, St. Louis, Missouri 63110.
Invest Ophthalmol Vis Sci. 1994 Jun;35(7):3085-93.
To examine conditions that determine the nature of the blood-borne, ACAID-inducing signal produced after intracameral injection of antigen.
Balb/c mice were splenectomized, rested, and injected in the anterior chamber with various antigens. Two days later the animals were bled, the plasma and white cells were isolated, and these fractions were transferred to naive mice (with spleens). Recipients were immunized subcutaneously within 2 to 7 days and delayed type hypersensitivity was assessed 10 to 14 days after immunization by challenge with the appropriate antigen.
The antigens HSV-1, TNP-coupled cells, and P815 tumors cells induced a soluble ACAID-inducing signal found in the plasma portion of blood. The soluble protein antigens bovine serum albumin (BSA) and conalbumin induced a cell-associated signal. When T-cells were included with protein antigens, a soluble (not cellular) ACAID-inducing signal was induced.
Particulate antigens, such as HSV-1 and P815, that elicit intraocular T-cell responses or antigens that contain T-cells (e.g., TNP cells) induce a soluble, ACAID-inducing signal. Soluble antigens (e.g., BSA and conalbumin) induce a cell-associated ACAID signal. Additionally, T-cells are capable of modulating the type of ACAID signal produced. These results show that two methods of delivering the ACAID signal exist that are dependent on the nature of the antigen and the presence of T-cells. The authors conclude that the eye shows great versatility in regulating potentially damaging immune responses.
研究决定前房内注射抗原后产生的血源性、诱导ACAID信号性质的条件。
对Balb/c小鼠进行脾切除，使其恢复，然后在前房内注射各种抗原。两天后采集动物血液，分离血浆和白细胞，并将这些组分转移至未接触过抗原的小鼠（有脾脏）体内。在2至7天内对受体小鼠进行皮下免疫，并在免疫后10至14天通过用相应抗原激发来评估迟发型超敏反应。
抗原单纯疱疹病毒1型（HSV-1）、三硝基苯（TNP）偶联细胞和P815肿瘤细胞诱导出一种存在于血液血浆部分的可溶性ACAID诱导信号。可溶性蛋白质抗原牛血清白蛋白（BSA）和伴清蛋白诱导出一种细胞相关信号。当蛋白质抗原与T细胞一起存在时，诱导出一种可溶性（而非细胞性）ACAID诱导信号。
引发眼内T细胞反应的颗粒性抗原，如HSV-1和P815，或含有T细胞的抗原（如TNP细胞）诱导出一种可溶性的ACAID诱导信号。可溶性抗原（如BSA和伴清蛋白）诱导出一种细胞相关的ACAID信号。此外，T细胞能够调节所产生的ACAID信号的类型。这些结果表明存在两种传递ACAID信号的方法，这取决于抗原的性质和T细胞的存在。作者得出结论，眼睛在调节潜在的有害免疫反应方面具有很大的灵活性。
