Massó F, Sandoval S, Rosas P, Paéz A, Díaz de León L, Zenteno E, Montaño L F
Departamento de Biología Celular, Instituto Nacional de Cardiología, México, D.F., Mexico.
Rev Latinoam Microbiol. 1993 Apr-Jun;35(2):177-84.
We present the results of an enzyme-linked immunosorbent assay that detects serum antibodies to M. tuberculosis antigens. The patients sera were tested with two different antigens, one a M. tuberculosis H37Rv culture filtrate protein extract precipitated with ammonium sulfate (CFPE), and the second, a delipidized CFPE (CFPE-d). The results obtained with 52 clearly defined TBP patients was 0.50 +/- 0.16 (CFPE) and 0.38 +/- 0.11 (CFPE-d). We also tested 165 sera from patients hospitalized at the INER with a diagnosis of non-tuberculous pulmonary disease. In this patients the assay revealed 153 negative sera (CFPE = 0.053 +/- 0.01; CFPE-d 0 0.050 +/- 0.004) and 12 positive sera. Nine of these patients (CFPE = 0.38 +/- 0.08; CFPE-d = 0.32 +/- 0.09) had mycobacterias and the results of the remaining three were considered as false positive. TBP was excluded in all the 153 negative patients. The elimination of lipids in the CFPE did not alter the assay since the differences between CFPE and CFPE-d did not reach statistical significance. In non-tuberculous individuals the reactivity with mycobacterial lipids was poor and didn't induce false positive results. The assay we report can be useful in those patients with pulmonary disease where TBP has to be considered.
我们展示了一种酶联免疫吸附测定的结果，该测定可检测针对结核分枝杆菌抗原的血清抗体。对患者血清用两种不同抗原进行检测，一种是用硫酸铵沉淀的结核分枝杆菌H37Rv培养滤液蛋白提取物（CFPE），另一种是脱脂CFPE（CFPE-d）。52例明确诊断的结核病患者的检测结果为CFPE为0.50±0.16，CFPE-d为0.38±0.11。我们还检测了165例在国家呼吸系统疾病研究所住院、诊断为非结核性肺病患者的血清。在这些患者中，该检测显示153例血清为阴性（CFPE = 0.053±0.01；CFPE-d = 0.050±0.004），12例血清为阳性。其中9例患者（CFPE = 0.38±0.08；CFPE-d = 0.32±0.09）有分枝杆菌，其余3例结果被视为假阳性。所有153例阴性患者均排除结核病。CFPE中脂质的去除未改变检测结果，因为CFPE与CFPE-d之间的差异未达到统计学显著性。在非结核个体中，与分枝杆菌脂质的反应性较差，未诱导出假阳性结果。我们报告的该检测方法对那些必须考虑结核病的肺病患者可能有用。
