Tobler K, Bridgen A, Ackermann M
Institute for Virology, Veterinary Medical Faculty, University of Zürich.
Adv Exp Med Biol. 1993;342:49-54. doi: 10.1007/978-1-4615-2996-5_8.
The nucleotide (nt) sequence of 1.7 kbp cDNA representing the 3' end of the PEDV genome has been determined. Viral RNA was reverse transcribed and the cDNA was amplified by polymerase chain reaction using degenerate primers. The sequences of the primers were based on conserved regions of coronaviral genomes. A 1323 nt open reading frame (ORF) showed good homology to the nucleocapsid (N) gene of other coronaviruses. The greatest homologies at the amino acid and the nt levels were observed with Human Coronavirus 229E. A second 336 nt ORF, which might encode a leucine-rich protein, was found within the N gene. Between the 3' end of the N gene and the poly(A) tail was a sequence of eleven nt, which is conserved among the other sequenced coronaviruses. Finally, a seven base sequence similar to the conserved intergenic sequences was present 5' to the N gene. These results confirm the classification of PEDV as a coronavirus.
已确定代表猪流行性腹泻病毒（PEDV）基因组3'端的1.7kbp cDNA的核苷酸（nt）序列。病毒RNA经逆转录，使用简并引物通过聚合酶链反应扩增cDNA。引物序列基于冠状病毒基因组的保守区域。一个1323nt的开放阅读框（ORF）与其他冠状病毒的核衣壳（N）基因具有良好的同源性。在氨基酸和核苷酸水平上，与人类冠状病毒229E的同源性最高。在N基因内发现了第二个336nt的ORF，它可能编码一种富含亮氨酸的蛋白质。在N基因的3'端和聚腺苷酸（poly(A)）尾之间有一个11nt的序列，在其他已测序的冠状病毒中是保守的。最后，在N基因的5'端存在一个与保守基因间序列相似的七碱基序列。这些结果证实了PEDV作为冠状病毒的分类。
