通过微量中和试验对高危人群中单纯疱疹病毒2型血清流行率的低估。

Underestimation of HSV-2 seroprevalence in a high-risk population by microneutralization assay.

作者信息

Ashley R L, Dalessio J, Dragavon J, Koutsky L A, Lee F K, Nahmias A J, Stevens C E, Holmes K K, Corey L

机构信息

Department of Laboratory Medicine, School of Medicine, University of Washington.

出版信息

Sex Transm Dis. 1993 Jul-Aug;20(4):230-5. doi: 10.1097/00007435-199307000-00009.

DOI:10.1097/00007435-199307000-00009

PMID:8211541

Abstract

BACKGROUND

Complement independent neutralizing antibody assays (CINA) have been used in seroepidemiologic studies and in diagnostic laboratories to distinguish between antibodies to herpes simplex virus types 1 (HSV-1) and 2 (HSV-2). The accuracy of CINA has not been rigorously tested against protein-specific typing assays, such as Western blot.

GOAL OF THIS STUDY

To determine the ability of CINA to identify HSV-2 antibodies alone or in the presence of HSV-1 antibodies.

STUDY DESIGN

Sera from randomly selected women at the Seattle King County Sexually Transmitted Disease Clinic were tested by CINA and Western blot.

RESULTS

Of 521 women tested, 81% had HSV antibodies by Western blot and 76% had neutralizing antibodies. Of 220 sera with HSV-2 antibodies by Western blot, 106 (48%) were serotyped correctly by CINA. Of the women studied, 140 (27%) had type-indeterminate neutralizing antibodies; 55 of these sera (39%) had antibody only to HSV-1 by Western blot.

CONCLUSION

The seroprevalence of HSV-2 in an STD clinic population was seriously underestimated by CINA.

摘要

背景

补体非依赖中和抗体检测（CINA）已用于血清流行病学研究及诊断实验室，以区分抗1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）的抗体。CINA的准确性尚未与蛋白质特异性分型检测（如免疫印迹法）进行严格对比测试。

本研究目的

确定CINA单独识别HSV-2抗体或在存在HSV-1抗体时识别HSV-2抗体的能力。

研究设计

对西雅图金县性传播疾病诊所随机选取的女性血清进行CINA和免疫印迹法检测。

结果

在接受检测的521名女性中，免疫印迹法显示81%的女性有HSV抗体，76%的女性有中和抗体。在免疫印迹法检测出有HSV-2抗体的220份血清中，CINA正确分型106份（48%）。在研究的女性中，140名（27%）有无法确定型别的中和抗体；其中55份血清（39%）免疫印迹法显示仅对HSV-1有抗体。