Wutzler P, Doerr H W, Färber I, Eichhorn U, Helbig B, Sauerbrei A, Brandstädt A, Rabenau H F
Institute for Antiviral Chemotherapy, Friedrich-Schiller University of Jena, Jena, Germany.
J Med Virol. 2000 Jun;61(2):201-7. doi: 10.1002/(sici)1096-9071(200006)61:2<201::aid-jmv5>3.0.co;2-p.
This study was carried out to determine the prevalence of antibodies to herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) in selected German populations, such as blood donors, hospital patients, and human immunodeficiency virus (HIV)-seropositive individuals. Serum samples collected between 1996 and 1998 were tested by enzyme immunoassays using monoclonal antibody-selected native gG1 and gG2 as antigens and an immunoblot using type-specific recombinant glycoproteins. Equivocal results were resolved by an "in-house" Western blot assay. The prevalence of HSV-1 antibodies increased steadily with age and reached high levels of >/=88% among subjects 40 years of age or older. In the sample of patients and blood donors, the HSV-2 seroprevalence was 12.8% (95% CI = 11.9-13.8%). About 81% of the HSV-2 seropositive subjects were coinfected with HSV-1. When adjusted for age, there was no difference in the HSV-2 seroprevalence between hospital patients and blood donors. The HSV-2 seroprevalence was significantly higher among women (15%) than among men (10.5%), yielding a female : male odds ratio of 1.5 for hospital patients and of 1.67 for blood donors. Among the HIV-infected population, 91.1% were seropositive for HSV-1 and 47.9% for HSV-2. HIV-infected women have a significantly higher risk of HSV-2 infection than men (odds ratio [OR] = 3.22; 95% confidence ratio [CI] 1.99-5.20). In conclusion, although the rate of infections with HSV-2 is relatively low in the German population, attention should be given to the further development in adolescents, especially in view of a possible decrease of HSV-1 seroprevalence in childhood.
本研究旨在确定德国特定人群中单纯疱疹病毒1型（HSV-1）和2型（HSV-2）抗体的流行情况，这些人群包括献血者、医院患者以及人类免疫缺陷病毒（HIV）血清反应阳性个体。对1996年至1998年间采集的血清样本进行检测，采用以单克隆抗体选择的天然gG1和gG2为抗原的酶免疫测定法，以及使用型特异性重组糖蛋白的免疫印迹法。通过“内部”蛋白质印迹法解决了模糊的结果。HSV-1抗体的流行率随年龄稳步上升，在40岁及以上的受试者中达到了≥88%的高水平。在患者和献血者样本中，HSV-2血清阳性率为12.8%（95%置信区间=11.9-13.8%）。约81%的HSV-2血清阳性受试者同时感染了HSV-1。在对年龄进行调整后，医院患者和献血者之间的HSV-2血清阳性率没有差异。女性的HSV-2血清阳性率（15%）显著高于男性（10.5%），医院患者的女性与男性优势比为1.5，献血者为1.67。在HIV感染人群中，91.1%的人HSV-1血清反应阳性，47.9%的人HSV-2血清反应阳性。HIV感染女性感染HSV-2的风险显著高于男性（优势比[OR]=3.22；95%置信区间[CI]1.99-5.20）。总之，尽管德国人群中HSV-2感染率相对较低，但应关注青少年中的进一步发展情况，特别是考虑到儿童期HSV-1血清阳性率可能下降。
