Bódis J, Tinneberg H R, Papenfuss F, Török A, Cledon P, Hanf V, Schwarz H
University Medical School of Pécs, Hungary.
Gynecol Endocrinol. 1993 Jun;7(2):83-7. doi: 10.3109/09513599309152485.
Cholinergic effects on hormone secretion by human granulosa cells (GCs) are not well characterized. The aim of this study was to explore the direct action of acetylcholine and carbachol on progesterone and estradiol secretion of human GCs cultured in serum-free medium. Granulosa cells were obtained from 26 women undergoing in vitro fertilization and embryo transfer. Progesterone and estradiol production was measured in the presence and absence of acetylcholine, carbachol, or atropine using radioimmunoassays; statistical analysis of the data was performed by ANOVA. Acetylcholine significantly stimulated progesterone secretion by GCs in a dose-related manner. Estradiol secretion was also stimulated by acetylcholine, but this effect did not show dose dependency. Carbachol showed a similar stimulatory effect, but to a lower degree; both effects can be blocked by acetylcholine. The results suggest that cholinergic action on steroid production by human GCs is mediated through the muscarinic route, and cholinergic neurotransmission may have a physiological significance in the intra-ovarian regulatory pathways.
胆碱能对人颗粒细胞（GCs）激素分泌的影响尚未得到充分表征。本研究的目的是探讨乙酰胆碱和卡巴胆碱对在无血清培养基中培养的人颗粒细胞孕酮和雌二醇分泌的直接作用。颗粒细胞取自26名接受体外受精和胚胎移植的女性。使用放射免疫分析法在有或无乙酰胆碱、卡巴胆碱或阿托品的情况下测量孕酮和雌二醇的产生；数据的统计分析采用方差分析。乙酰胆碱以剂量相关的方式显著刺激颗粒细胞孕酮分泌。乙酰胆碱也刺激雌二醇分泌，但这种作用不显示剂量依赖性。卡巴胆碱显示出类似的刺激作用，但程度较低；两种作用均可被阿托品阻断。结果表明，胆碱能对人颗粒细胞类固醇生成的作用是通过毒蕈碱途径介导的，胆碱能神经传递可能在卵巢内调节途径中具有生理意义。
