Bódis J, Tinneberg H R, Schwarz H, Papenfuss F, Török A, Hanf V
University Women's Hospital of Tübingen, Germany.
Gynecol Endocrinol. 1993 Dec;7(4):235-9. doi: 10.3109/09513599309152507.
The aim of this study was to explore the direct action of histamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Human granulosa cells were isolated from preovulatory follicular fluid aspirated from 17 women (32 +/- 3 years old, mean +/- SD) undergoing in vitro fertilization treatment at the University Women's Hospital of Tübingen. Progesterone and estradiol production was measured in the presence and absence of histamine, terfenadine or cimetidine using radioimmunoassays. Statistical analysis of the data was performed by analysis of variance and Newmann-Keul tests. Histamine stimulated a dose-related increase in estradiol secretion with a maximal stimulatory effect at 10(-3) mol/l. This response was blocked specifically by the H1-receptor antagonist terfenadine. Progesterone production in response to histamine stimulation was independent of dose at the limit of significance. The specific H2-receptor antagonist cimetidine did not block the stimulatory effect of histamine. We suggest that histamine has a direct stimulatory effect on steroid production of granulosa cells mediated via the H1-receptor. This effect may have a physiological role in the regulation of granulosa cell function during the menstrual cycle.
本研究的目的是探讨组胺对在无血清培养基中培养的人颗粒细胞孕酮和雌二醇分泌的直接作用。从图宾根大学妇女医院接受体外受精治疗的17名女性（32±3岁，平均±标准差）抽吸的排卵前卵泡液中分离出人颗粒细胞。使用放射免疫分析法在有或无组胺、特非那定或西咪替丁的情况下测量孕酮和雌二醇的产生。通过方差分析和纽曼-库尔检验对数据进行统计分析。组胺刺激雌二醇分泌呈剂量相关增加，在10(-3)mol/L时具有最大刺激作用。这种反应被H1受体拮抗剂特非那定特异性阻断。在显著水平上，组胺刺激引起的孕酮产生与剂量无关。特异性H2受体拮抗剂西咪替丁并未阻断组胺的刺激作用。我们认为组胺对颗粒细胞类固醇产生具有直接刺激作用，通过H1受体介导。这种作用可能在月经周期中颗粒细胞功能的调节中具有生理作用。
