Bódis J, Tinneberg H R, Török A, Cledon P, Hanf V, Papenfuss F
University Women's Hospital of Tübingen, Germany.
Acta Endocrinol (Copenh). 1993 Aug;129(2):165-8. doi: 10.1530/acta.0.1290165.
The aim of this study was to explore the direct action of noradrenaline and dopamine on progesterone and estradiol secretion of human granulosa cells cultured in serum-free medium. Progesterone and estradiol production was measured in the presence and absence of noradrenaline, dopamine or propranolol using radioimmunoassays; statistical analysis was performed by analysis of variance and Newman-Keul's multiple range test. Twenty-six women aged 31 +/- 3 years undergoing in vitro fertilization and embryo transfer for infertility treatment at University Women's Hospital, University of Tübingen, Germany, took part in this study. Noradrenaline significantly inhibited progesterone production by human granulosa cells in a dose-related manner at a concentration of 10(-4)-10(-6) mol/l. Dopamine significantly stimulated estradiol secretion by granulosa cells in an inverse dose-related manner. Both effects were blocked by propranolol. The results suggest that catecholaminergic actions switch over the steroid production of human granulosa cells cultured in serum-free medium from progesterone to estradiol.
本研究旨在探讨去甲肾上腺素和多巴胺对在无血清培养基中培养的人颗粒细胞孕酮和雌二醇分泌的直接作用。使用放射免疫分析法测定有无去甲肾上腺素、多巴胺或普萘洛尔时孕酮和雌二醇的生成；采用方差分析和纽曼-库尔多重范围检验进行统计分析。26名年龄在31±3岁的女性参与了本研究，她们因不孕症在德国图宾根大学妇女医院接受体外受精和胚胎移植治疗。去甲肾上腺素在浓度为10(-4)-10(-6)mol/l时以剂量相关方式显著抑制人颗粒细胞的孕酮生成。多巴胺以剂量反向相关方式显著刺激颗粒细胞的雌二醇分泌。两种作用均被普萘洛尔阻断。结果表明，儿茶酚胺能作用使在无血清培养基中培养的人颗粒细胞的类固醇生成从孕酮转换为雌二醇。
