Nilsson-Ehle I, Yoshikawa T T, Schotz M C, Guze L B
Antimicrob Agents Chemother. 1976 May;9(5):754-60. doi: 10.1128/AAC.9.5.754.
A method for quantitative determination of tetracycline in serum, using high-pressure liquid chromatography, is reported. After extraction of the drug from serum, using methanol-trichloroacetic acid solution, tetracycline was separated by reverse-phase chromatography. Quantitation of tetracycline was based on a linear relationship between peak heights in the chromatograms and known concentrations of the drug in the original serum samples. Serum tetracycline concentrations as low as 0.3 mug/ml could be accurately measured. Serum samples obtained after a single intravenous injection of tetracycline to three human volunteers and six dogs were assayed by microbiological and chemical assays. Correlation coefficients of 0.95 and 0.97, respectively, were found. This chemical method is rapid (less than 30 min), accurate, sensitive, and reproducible, and it seems feasible for routine clinical use.
报道了一种采用高压液相色谱法定量测定血清中四环素的方法。用甲醇 - 三氯乙酸溶液从血清中提取药物后,通过反相色谱法分离四环素。四环素的定量基于色谱图中峰高与原始血清样品中已知药物浓度之间的线性关系。血清中低至0.3微克/毫升的四环素浓度都能被准确测定。对三名人类志愿者和六只狗单次静脉注射四环素后获得的血清样本进行了微生物学和化学分析。分别发现相关系数为0.95和0.97。这种化学方法快速(少于30分钟)、准确、灵敏且可重复,似乎适用于常规临床应用。