Winkler H H
Appl Environ Microbiol. 1976 Jan;31(1):146-9. doi: 10.1128/aem.31.1.146-149.1976.
A micro space technique for determining membrane permeability in Rickettsia prowazeki is described and justified. The cell water, cell wall plus periplasmic volume, and glutamate, ethylene glycol, and adenosine diphosphate permeabilities were determined by this method. The effect of nonionic detergents on rickettsial permeability was examined: Triton X-100 destroyed the permeability barrier, whereas Lubrol-WX left it intact.
描述并论证了一种用于测定普氏立克次氏体膜通透性的微空间技术。通过该方法测定了细胞内水、细胞壁加周质体积以及谷氨酸、乙二醇和二磷酸腺苷的通透性。研究了非离子洗涤剂对立克次氏体通透性的影响:Triton X-100破坏了通透性屏障,而Lubrol-WX使其保持完整。