苯并芘和黄樟素对联苯2-羟化酶及其他药物代谢酶的影响。
The effects of benzopyrene and safrole on biphenyl 2-hydroxylase and other drug-metabolizing enzymes.
作者信息
McPherson F J, Bridges J W, Parke D V
出版信息
Biochem J. 1976 Mar 15;154(3):773-80. doi: 10.1042/bj1540773.
A study was made of the nature and specificity of the increase in biphenyl 2-hydroxylase activity after preincubation of liver microsomal preparations with various carcinogens in vitro. This enhancement of enzyme activity in vitro was investigated in mouse, hamster and rat, and although the rat appears to be atypical in the variation of the pattern of 2- and 4-hydroxylation with age, similar enhancements were detectable in each species examined. An increase in biphenyl 2-hydroxylase activity was apparent 2h after intraperitoneal administration of safrole or benzopyrene to mature Wistar albino rats and appeared to be similar in nature to that observed after preincubation of liver microsomal preparations with the same chemical in vitro. Investigation of other drug-metabolizing enzyme systems suggests that the enhancing effects of carcinogens in vitro are specific for biphenyl 2-hydroxylase. No correlation between the enhancement of biphenyl 2-hydroxylase and inhibtion of biphenyl 4-hydroxylase was apparent.
对肝微粒体制剂在体外与各种致癌物预孵育后联苯2-羟化酶活性增加的性质和特异性进行了研究。在小鼠、仓鼠和大鼠中研究了这种体外酶活性的增强情况，尽管大鼠在2-羟化和4-羟化模式随年龄变化方面似乎不典型，但在所检查的每个物种中都可检测到类似的增强。给成年Wistar白化大鼠腹腔注射黄樟素或苯并芘后2小时，联苯2-羟化酶活性明显增加，其性质似乎与肝微粒体制剂在体外与相同化学物质预孵育后观察到的相似。对其他药物代谢酶系统的研究表明，致癌物在体外的增强作用对联苯2-羟化酶具有特异性。联苯2-羟化酶的增强与联苯4-羟化酶的抑制之间没有明显的相关性。
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