利用李斯特菌噬菌体和季铵化合物对黏附的单核细胞增生李斯特菌进行生物灭活。
Biological inactivation of adhering Listeria monocytogenes by listeriaphages and a quaternary ammonium compound.
作者信息
Roy B, Ackermann H W, Pandian S, Picard G, Goulet J
机构信息
Dairy Research Center (STELA), Faculty of Agriculture, Université Laval, Ste-Foy, Québec, Canada.
出版信息
Appl Environ Microbiol. 1993 Sep;59(9):2914-7. doi: 10.1128/aem.59.9.2914-2917.1993.
Abstract
The use of listeriaphages as a means of disinfecting contaminated stainless-steel and polypropylene surfaces was investigated. Surfaces artificially contaminated with L. monocytogenes 10401 and 8427 were sanitized with suspensions of listeriaphages (H387, H387-A, and 2671), all belonging to the Siphoviridae family. Phage suspensions at concentrations of up to 3.5 x 10(8) PFU/ml were at least as efficient as a 20 ppm solution of a quaternary ammonium compound (QUATAL) in reducing L. monocytogenes populations. A synergistic activity was observed when two or more phages were used in combination and when phages were suspended in QUATAL. The biological activity of the three phages was not affected by QUATAL concentrations of 50 ppm and a contact time of 4 h.
摘要
研究了利用李斯特菌噬菌体对受污染的不锈钢和聚丙烯表面进行消毒的方法。用均属于长尾噬菌体科的李斯特菌噬菌体（H387、H387 - A和2671）悬浮液对人工污染了单核细胞增生李斯特菌10401和8427的表面进行消毒。浓度高达3.5×10⁸ PFU/ml的噬菌体悬浮液在减少单核细胞增生李斯特菌数量方面至少与20 ppm的季铵化合物（QUATAL）溶液一样有效。当两种或更多种噬菌体联合使用以及噬菌体悬浮于QUATAL中时，观察到了协同活性。50 ppm的QUATAL浓度和4小时的接触时间对这三种噬菌体的生物活性没有影响。
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