Lacoste A M, Cassaigne A, Tamari M, Neuzil E
Biochimie. 1976;58(6):703-12. doi: 10.1016/s0300-9084(76)80395-1.
2-Aminoethylphosphonic acid (ciliatine) can be used as a source of phosphorus or nitrogen by Pseudomonas aeruginosa. The conditions of its uptake have been investigated. The transport is inducible by ciliatine itself or by its homologue, 3-aminopropylphosphonate, but neither by other phosphonic compounds nor by carboxylic or sulfonic related derivatives. The induction was not suppressed by inorganic phosphate. The transport appears to be an active process, pH and temperature dependent: it requires energy and is dependent on new protein synthesis. The uptake follows Michaelis kinetics. The substrate specificity involved in ciliatine uptake favours the existence of two different transport systems: the first one, inducible by ciliatine, was very sensitive towards different aminophosphonic acids and was competitively inhibited by inorganic phosphate and methylphosphonate; the second transport system, inducible by 3-amino-propylphosphonate, appeared less sensitive towards alpha-aminophosphonic acids and was non competitively inhibited by phosphate and methylphosphonate. No interactions were observed with related aminocarboxylic acids or with taurine. Some molecular structural requirements for the binding of an effector on both permeases are discussed. The regulatory function of inorganic phosphate, the chief breakdown product of ciliatine, is also emphasized.
2-氨基乙基膦酸（纤毛素）可被铜绿假单胞菌用作磷或氮的来源。对其摄取条件进行了研究。转运可由纤毛素本身或其同系物3-氨基丙基膦酸诱导，但不能由其他膦酸化合物或羧酸或磺酸相关衍生物诱导。无机磷酸盐不会抑制这种诱导作用。转运似乎是一个活跃的过程，依赖于pH值和温度：它需要能量，并且依赖于新的蛋白质合成。摄取遵循米氏动力学。纤毛素摄取所涉及的底物特异性有利于两种不同转运系统的存在：第一种由纤毛素诱导，对不同的氨基膦酸非常敏感，并被无机磷酸盐和甲基膦酸竞争性抑制；第二种转运系统由3-氨基丙基膦酸诱导，对α-氨基膦酸的敏感性较低，并且被磷酸盐和甲基膦酸非竞争性抑制。未观察到与相关氨基羧酸或牛磺酸的相互作用。讨论了效应物与两种通透酶结合的一些分子结构要求。还强调了纤毛素的主要分解产物无机磷酸盐的调节功能。
