草甘膦被节杆菌属吸收
Uptake of Glyphosate by an Arthrobacter sp.
机构信息
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D 4630 Bochum, Federal Republic of Germany.
出版信息
Appl Environ Microbiol. 1987 May;53(5):974-8. doi: 10.1128/aem.53.5.974-978.1987.
Abstract
The uptake of glyphosate (N-[phosphonomethyl]glycine) by an Arthrobacter sp. which can utilize this herbicide as its sole source of phosphorus was investigated. Orthophosphate suppressed the expression of the uptake system for glyphosate and also competed with glyphosate for uptake. The K(m) for glyphosate uptake was 125 muM, and the K(i) for orthophosphate was 24 muM. Organophosphonates as well as organophosphates inhibited glyphosate uptake, but only organophosphates and orthophosphate suppressed the uptake system. Glyphosate uptake was energy dependent, had a pH optimum of 6 to 7, and was differentially affected by divalent cations.
摘要
研究了一株能够将草甘膦（N-[膦酸甲基]甘氨酸）用作唯一磷源的节杆菌对草甘膦的吸收情况。正磷酸盐抑制了草甘膦摄取系统的表达，并与草甘膦竞争摄取。草甘膦摄取的 K(m)为 125 μM，K(i)为正磷酸盐 24 μM。有机膦酸酯以及有机磷酸酯抑制草甘膦摄取，但只有有机磷酸酯和正磷酸盐抑制摄取系统。草甘膦摄取依赖于能量，最适 pH 值为 6 到 7，并且受到二价阳离子的差异影响。
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本文引用的文献
1
Selection and characterization of a carrot cell line tolerant to glyphosate.一种耐草甘膦胡萝卜细胞系的筛选与鉴定
Plant Physiol. 1984 Nov;76(3):571-4. doi: 10.1104/pp.76.3.571.
2
Uptake and distribution of N-phosphonomethylglycine in sugar beet plants.草甘膦在甜菜植株中的吸收与分布。
Plant Physiol. 1981 Sep;68(3):668-72. doi: 10.1104/pp.68.3.668.
3
Effect of Glyphosate on Intact Bean Plants (Phaseolus vulgaris L.) and Isolated Cells.草甘膦对完整菜豆植株（Phaseolus vulgaris L.）和分离细胞的影响。
Plant Physiol. 1980 Oct;66(4):656-9. doi: 10.1104/pp.66.4.656.
4
Glyphosate-degrading microorganisms from industrial activated sludge.来自工业活性污泥的降解草甘膦的微生物。
Appl Environ Microbiol. 1986 Feb;51(2):432-4. doi: 10.1128/aem.51.2.432-434.1986.
5
Phosphonate Utilization by the Glyphosate-Degrading Pseudomonas sp. Strain PG2982.利用抗草甘膦的恶臭假单胞菌 PG2982 菌株进行膦酸盐的利用。
Appl Environ Microbiol. 1984 Nov;48(5):1049-50. doi: 10.1128/aem.48.5.1049-1050.1984.
6
Isolation of a Pseudomonas sp. Which Utilizes the Phosphonate Herbicide Glyphosate.一株利用膦酸类除草剂草甘膦的假单胞菌的分离。
Appl Environ Microbiol. 1983 Aug;46(2):316-20. doi: 10.1128/aem.46.2.316-320.1983.
7
Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.无机磷酸盐对大肠杆菌中磷酸酶形成的影响。
Biochim Biophys Acta. 1960 Mar 11;38:460-9. doi: 10.1016/0006-3002(60)91281-6.
8
A possible negative feedback phenomenon controlling formation of alkaline phosphomonoesterase in Escherichia coli.一种可能控制大肠杆菌中碱性磷酸单酯酶形成的负反馈现象。
Nature. 1959 May 30;183(4674):1529-30. doi: 10.1038/1831529b0.
9
Characterization of two genetically separable inorganic phosphate transport systems in Escherichia coli.大肠杆菌中两种基因可分离的无机磷酸盐转运系统的特性分析。
J Bacteriol. 1980 Oct;144(1):356-65. doi: 10.1128/jb.144.1.356-365.1980.
10
5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae. 1. Purification and properties.肺炎克雷伯菌的5-烯醇丙酮酸莽草酸-3-磷酸合酶。1. 纯化及性质
Eur J Biochem. 1984 Sep 3;143(2):341-9. doi: 10.1111/j.1432-1033.1984.tb08378.x.
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