一株利用草甘膦作为唯一磷源和氮源的节杆菌 GLP-1 突变株的分离和鉴定。
Isolation and Characterization of a Mutant of Arthrobacter sp. Strain GLP-1 Which Utilizes the Herbicide Glyphosate as Its Sole Source of Phosphorus and Nitrogen.
机构信息
Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D-4630 Bochum, Federal Republic of Germany.
出版信息
Appl Environ Microbiol. 1988 Nov;54(11):2868-70. doi: 10.1128/aem.54.11.2868-2870.1988.
Abstract
Arthrobacter sp. strain GLP-1, grown on glucose as a carbon source, utilizes the herbicide glyphosate [N-(phosphonomethyl)glycine] as its sole source of phosphorus as well as its sole source of nitrogen. The mutant strain GLP-1/Nit-1 utilizes glyphosate as its sole source of nitrogen as well. In strain GLP-1, P(i) was a potent competitive inhibitor of glyphosate uptake (K(i), 24 muM), while the affinity of P(i) for the uptake system of strain GLP-1/Nit-1 was reduced by 2 orders of magnitude (K(i), 2.3 mM). It is concluded that the inability of strain GLP-1 to utilize glyphosate as a source of nitrogen is due to the stringent control of glyphosate uptake by excess phosphate released during the degradation of the herbicide.
摘要
节杆菌 GLP-1 菌株以葡萄糖作为碳源，利用除草剂草甘膦（N-（膦酸甲基）甘氨酸）作为其唯一的磷源和氮源。突变株 GLP-1/Nit-1 也将草甘膦用作其唯一的氮源。在 GLP-1 菌株中，Pi 是草甘膦摄取的强竞争性抑制剂（Ki，24 μM），而 Pi 对 GLP-1/Nit-1 菌株摄取系统的亲和力降低了 2 个数量级（Ki，2.3 mM）。结论是，GLP-1 菌株不能将草甘膦用作氮源是由于在除草剂降解过程中释放的过量磷酸盐对草甘膦摄取的严格控制。
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