C类β-内酰胺酶催化的β-内酰胺水解非化学计量爆发动力学。
The kinetics of non-stoichiometric bursts of beta-lactam hydrolysis catalysed by class C beta-lactamases.
作者信息
Page M G
机构信息
Pharma Division, Preclinical Research, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
出版信息
Biochem J. 1993 Oct 1;295 ( Pt 1)(Pt 1):295-304. doi: 10.1042/bj2950295.
Abstract
Class C beta-lactamases from Pseudomonas aeruginosa and several species of the Enterobacteriaceae have been observed to undergo a rapid burst in hydrolysis of beta-lactam antibiotics before relaxation to a steady-state rate of hydrolysis. The amplitude of the burst corresponds to the hydrolysis of between 1 and 10,000 mol of the substrate per mol of enzyme. The decay of the rate of hydrolysis in the burst phase comprises two exponential reactions, which indicates that there are three different reactive states of the enzymes. Examination of the kinetics of acylation by slowly reacting beta-lactams suggests that there are three forms of the free enzyme in slow equilibrium. Thus it would appear that the burst kinetics exhibited by class C enzymes can be attributed to redistribution of the enzyme between different conformations induced by the reaction with substrate.
摘要
已观察到铜绿假单胞菌和几种肠杆菌科细菌的C类β-内酰胺酶在弛豫到稳定水解速率之前，会对β-内酰胺抗生素进行快速的水解爆发。爆发的幅度相当于每摩尔酶水解1至10,000摩尔底物。爆发阶段水解速率的衰减包括两个指数反应，这表明酶存在三种不同的反应状态。对缓慢反应的β-内酰胺进行酰化动力学研究表明，游离酶有三种形式处于缓慢平衡状态。因此，C类酶表现出的爆发动力学似乎可归因于酶在与底物反应诱导的不同构象之间的重新分布。
相似文献
1
The kinetics of non-stoichiometric bursts of beta-lactam hydrolysis catalysed by class C beta-lactamases.C类β-内酰胺酶催化的β-内酰胺水解非化学计量爆发动力学。
Biochem J. 1993 Oct 1;295 ( Pt 1)(Pt 1):295-304. doi: 10.1042/bj2950295.
2
Chromophoric spin-labeled beta-lactam antibiotics for ENDOR structural characterization of reaction intermediates of class A and class C beta-lactamases.用于A类和C类β-内酰胺酶反应中间体ENDOR结构表征的发色自旋标记β-内酰胺抗生素。
Spectrochim Acta A Mol Biomol Spectrosc. 2004 May;60(6):1279-89. doi: 10.1016/j.saa.2003.10.024.
3
A kinetic study of NMC-A beta-lactamase, an Ambler class A carbapenemase also hydrolyzing cephamycins.NMC-Aβ-内酰胺酶的动力学研究，一种也能水解头孢霉素的安布勒A类碳青霉烯酶。
FEMS Microbiol Lett. 1996 Sep 15;143(1):29-33. doi: 10.1111/j.1574-6968.1996.tb08457.x.
4
Interaction of beta-lactamases I and II from Bacillus cereus with semisynthetic cephamycins. Kinetic studies.蜡样芽孢杆菌β-内酰胺酶I和II与半合成头孢霉素的相互作用。动力学研究。
Biochem J. 1991 Oct 1;279 ( Pt 1)(Pt 1):111-4.
5
Role of asparagine 152 in catalysis of beta-lactam hydrolysis by Escherichia coli AmpC beta-lactamase studied by site-directed mutagenesis.
Biochemistry. 1995 Jun 13;34(23):7757-64. doi: 10.1021/bi00023a023.
6
Kinetics of Sulbactam Hydrolysis by β-Lactamases, and Kinetics of β-Lactamase Inhibition by Sulbactam.β-内酰胺酶对舒巴坦的水解动力学及舒巴坦对β-内酰胺酶的抑制动力学。
Antimicrob Agents Chemother. 2017 Nov 22;61(12). doi: 10.1128/AAC.01612-17. Print 2017 Dec.
7
Beta-lactamases as fully efficient enzymes. Determination of all the rate constants in the acyl-enzyme mechanism.β-内酰胺酶作为完全有效的酶。酰基酶机制中所有速率常数的测定。
Biochem J. 1990 Mar 15;266(3):853-61.
8
Interactions of "bora-penicilloates" with serine β-lactamases and DD-peptidases.“硼青霉素酯”与丝氨酸β-内酰胺酶和 DD-肽酶的相互作用。
Biochemistry. 2014 Oct 21;53(41):6530-8. doi: 10.1021/bi500970f. Epub 2014 Oct 10.
9
Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of beta-lactam antibiotics.青霉素酰化酶催化合成β-内酰胺抗生素过程中酶酰化与脱酰化的动力学
Eur J Biochem. 2003 Sep;270(18):3675-83. doi: 10.1046/j.1432-1033.2003.03728.x.
10
The role of tyrosine 150 in catalysis of beta-lactam hydrolysis by AmpC beta-lactamase from Escherichia coli investigated by site-directed mutagenesis.通过定点诱变研究酪氨酸150在大肠杆菌AmpC β-内酰胺酶催化β-内酰胺水解中的作用。
Biochemistry. 1994 Jul 19;33(28):8577-86. doi: 10.1021/bi00194a024.
引用本文的文献
1
Epistasis arises from shifting the rate-limiting step during enzyme evolution of a β-lactamase.上位效应源于在β-内酰胺酶的酶进化过程中改变限速步骤。
Nat Catal. 2024;7(5):499-509. doi: 10.1038/s41929-024-01117-4. Epub 2024 Feb 23.
2
Detection of an enzyme isomechanism by means of the kinetics of covalent inhibition.通过共价抑制的动力学检测酶的同工酶机制。
Biochim Biophys Acta Proteins Proteom. 2021 Sep;1869(9):140681. doi: 10.1016/j.bbapap.2021.140681. Epub 2021 Jun 2.
3
Bicyclic Boronates as Potent Inhibitors of AmpC, the Class C β-Lactamase from .双环硼酸酯作为 AmpC 的强效抑制剂，AmpC 是来自. 的 C 类β-内酰胺酶。
Biomolecules. 2020 Jun 12;10(6):899. doi: 10.3390/biom10060899.
4
A Unified Numbering Scheme for Class C β-Lactamases.C 类β-内酰胺酶的统一编号方案。
Antimicrob Agents Chemother. 2020 Feb 21;64(3). doi: 10.1128/AAC.02247-19.
5
Conformational Intermediate That Controls KPC-2 Catalysis and Beta-Lactam Drug Resistance.控制KPC-2催化作用及β-内酰胺类药物耐药性的构象中间体
ACS Catal. 2018 Apr 6;8(4):2741-2747. doi: 10.1021/acscatal.7b03832. Epub 2018 Feb 21.
6
Exposing a β-Lactamase "Twist": the Mechanistic Basis for the High Level of Ceftazidime Resistance in the C69F Variant of the Burkholderia pseudomallei PenI β-Lactamase.揭示β-内酰胺酶的“扭曲”：伯克霍尔德菌假鼻疽杆菌PenIβ-内酰胺酶C69F变体中高水平头孢他啶耐药性的机制基础。
Antimicrob Agents Chemother. 2015 Nov 23;60(2):777-88. doi: 10.1128/AAC.02073-15. Print 2016 Feb.
7
In vitro and in vivo properties of BAL30376, a β-lactam and dual beta-lactamase inhibitor combination with enhanced activity against Gram-negative Bacilli that express multiple β-lactamases.BAL30376 的体外和体内特性研究，这是一种β-内酰胺类药物和双重β-内酰胺酶抑制剂组合，对表达多种β-内酰胺酶的革兰氏阴性杆菌具有增强的活性。
Antimicrob Agents Chemother. 2011 Apr;55(4):1510-9. doi: 10.1128/AAC.01370-10. Epub 2011 Jan 18.
8
In vitro properties of BAL30072, a novel siderophore sulfactam with activity against multiresistant gram-negative bacilli.BAL30072 是一种新型的含磺胺的铁载体，对多重耐药革兰氏阴性杆菌具有活性。体外研究结果表明。
Antimicrob Agents Chemother. 2010 Jun;54(6):2291-302. doi: 10.1128/AAC.01525-09. Epub 2010 Mar 22.
9
Inhibition of the class C beta-lactamase from Acinetobacter spp.: insights into effective inhibitor design.抑制不动杆菌属的 C 类β-内酰胺酶：有效抑制剂设计的见解。
Biochemistry. 2010 Jan 19;49(2):329-40. doi: 10.1021/bi9015988.
10
Interactions of ceftobiprole with beta-lactamases from molecular classes A to D.头孢托罗与A至D类分子的β-内酰胺酶的相互作用。
Antimicrob Agents Chemother. 2007 Sep;51(9):3089-95. doi: 10.1128/AAC.00218-07. Epub 2007 Jun 25.
本文引用的文献
1
A method for characterizing the type and numbers of groups involved in enzyme action.一种表征参与酶作用的基团类型和数量的方法。
J Biol Chem. 1961 Jul;236:1973-9.
2
Hysteretic enzymes.滞后酶
Methods Enzymol. 1980;64:192-226. doi: 10.1016/s0076-6879(80)64010-5.
3
Inhibition of the RTEM beta-lactamase from Escherichia coli. Interaction of enzyme with derivatives of olivanic acid.
Biochemistry. 1981 May 12;20(10):2732-7. doi: 10.1021/bi00513a005.
4
The E. coli beta-lactamase attenuator mediates growth rate-dependent regulation.大肠杆菌β-内酰胺酶衰减子介导生长速率依赖性调控。
Nature. 1981 Mar 19;290(5803):221-5. doi: 10.1038/290221a0.
5
Mechanism of substrate-induced inactivation of beta-lactamase I.底物诱导β-内酰胺酶I失活的机制。
Eur J Biochem. 1980 Aug;109(2):575-80. doi: 10.1111/j.1432-1033.1980.tb04830.x.
6
Kinetics of protein modification reactions.蛋白质修饰反应的动力学
Biochem J. 1984 Jan 15;217(2):341-51. doi: 10.1042/bj2170341.
7
Purification of beta-lactamases by affinity chromatography on phenylboronic acid-agarose.通过苯基硼酸 - 琼脂糖亲和层析法纯化β - 内酰胺酶。
Biochem J. 1984 Jul 15;221(2):505-12. doi: 10.1042/bj2210505.
8
The reversible deactivation of beta-lactamase from Staphylococcus aureus by quinacillin and cephaloridine and its modification by antibodies.喹那西林和头孢菌素对金黄色葡萄球菌β-内酰胺酶的可逆失活作用及其抗体修饰
Biochim Biophys Acta. 1984 Mar 29;785(3):104-10. doi: 10.1016/0167-4838(84)90133-x.
9
The structure of beta-lactamases.β-内酰胺酶的结构
Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321-31. doi: 10.1098/rstb.1980.0049.
10
Equilibrium and rate constants for the interconversion of two conformations of -chymotrypsin. The existence of a catalytically inactive conformation at neutral p H.
J Mol Biol. 1971 Sep 14;60(2):279-90. doi: 10.1016/0022-2836(71)90294-4.
Zotero 插件