Bröker B M, Korthäuer U, Heppt P, Weseloh G, de la Camp R, Kroczek R A, Emmrich F
Max-Planck-Society, University of Erlangen-Nuremberg, Germany.
Arthritis Rheum. 1993 Sep;36(9):1234-43. doi: 10.1002/art.1780360908.
To analyze the T cell receptor (TCR) variable (V) region gene usage in the rheumatoid joint.
Monoclonal antibodies (MAb) were used to determine the prevalence of selected V elements on T cells in synovial fluid (SF) from rheumatoid arthritis (RA) patients and in peripheral blood (PB) from RA patients and normal controls. V alpha 2-positive PB and SF T cells from 1 patient were cloned by immediate limiting-dilution and analyzed by restriction mapping.
In 9 of 14 RA patients, SF was enriched in at least 1 of the selected V elements, compared with PB. TCR genes of the V alpha 2 family were the most frequently overrepresented in the SF (4 patients). The expanded V alpha 2-positive cells were oligoclonal in SF but heterogeneic in PB.
Our data showing biased and clonally restricted TCR elements in the rheumatoid joint indicate major histocompatibility complex-restricted antigen recognition, rather than a "superantigen," in the pathogenesis of RA.
分析类风湿关节中T细胞受体（TCR）可变（V）区基因的使用情况。
使用单克隆抗体（MAb）来确定类风湿关节炎（RA）患者滑液（SF）中以及RA患者和正常对照者外周血（PB）中T细胞上选定V元件的流行情况。通过立即有限稀释法克隆了1例患者的Vα2阳性PB和SF T细胞，并通过限制性图谱分析。
与PB相比，在14例RA患者中的9例中，SF中至少1种选定的V元件富集。Vα2家族的TCR基因在SF中最常过度表达（4例患者）。扩增的Vα2阳性细胞在SF中是寡克隆的，但在PB中是异质性的。
我们的数据显示类风湿关节中TCR元件存在偏向性且克隆受限，这表明在RA发病机制中主要组织相容性复合体限制的抗原识别，而非“超抗原”。
