Lopez Ivan, Ishiyama Gail, Tang Yong, Tokita Joshua, Baloh Robert W, Ishiyama Akira
Surgery Department, Division of Head and Neck, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.
J Neurosci Res. 2005 Nov 1;82(3):421-31. doi: 10.1002/jnr.20652.
Regional estimates of type I and type II vestibular hair cells (HC) and supporting cell (SC) numbers were obtained from the horizontal crista ampullaris by using design-based stereology in human. Cristae were microdissected from temporal bones obtained post-mortem (N=16, age range 26-98 years). Three groups were made according to age: group 1, n=5, ages between 26 and 67 years, average age 51 years; group 2, n=4, average age 84 years; and group 3, n=7, average age 94 years. For group 1, the average total HC number was 8,005+/-214, corresponding to 4,119+/-107 type I HC, 3,886+/-117 type II HC, and 10,274+/-224 SC. The type I:type II HC ratio was 1.06+/-0.01, and HC density was 0.80 cells/100 microm2. For group 2, the average total HC number was 7,074+/-489, corresponding to 3,733+/-212 type I HC, 3,341+/-314 type II HC, and 9,321+/-858 SC. The type I:II HC ratio was 1.12+/-0.06, and HC density was 0.75 cells/100 microm2. For group 3, the average HC number was 6,009+/-327, corresponding to 3,380+/-223 type I HC, 2,628+/-235 type II HC, and 10,185+/-182 SC. The type I:II HC ratio was 1.34+/-0.10, and HC density was 0.63 cells/100 microm2. A significant decline in type I, type II, and total HC number and density was found in groups 2 and 3, with individuals exceeding the average human life span.
通过基于设计的体视学方法，在人体水平壶腹嵴中获得了I型和II型前庭毛细胞（HC）以及支持细胞（SC）数量的区域估计值。从死后获取的颞骨中显微解剖出壶腹嵴（N = 16，年龄范围26 - 98岁）。根据年龄分为三组：第1组，n = 5，年龄在26至67岁之间，平均年龄51岁；第2组，n = 4，平均年龄84岁；第3组，n = 7，平均年龄94岁。对于第1组，平均总HC数量为8,005±214，对应4,119±107个I型HC、3,886±117个II型HC和10,274±224个SC。I型：II型HC比例为1.06±0.01，HC密度为0.80个细胞/100平方微米。对于第2组，平均总HC数量为7,074±489，对应3,733±212个I型HC、3,341±314个II型HC和9,321±858个SC。I型：II型HC比例为1.12±0.06，HC密度为0.75个细胞/100平方微米。对于第3组，平均HC数量为6,009±327，对应3,380±223个I型HC、2,628±235个II型HC和10,185±182个SC。I型：II型HC比例为1.34±0.10，HC密度为0.63个细胞/100平方微米。在第2组和第3组中，即年龄超过人类平均寿命的个体中，发现I型、II型和总HC数量及密度显著下降。
