Minami M, Huh G H, Yang P, Iwabuchi M
Department of Botany, Faculty of Science, Kyoto University, Japan.
Plant Mol Biol. 1993 Oct;23(2):429-34. doi: 10.1007/BF00029019.
The expression of genes encoding five histones (H1, H2A, H2B, H3 and H4) and the putative transcription factors HBP-1a (17) and HBP-1b (c38) was examined during early germination and in various tissues of young wheat seedlings. The steady-state levels of core histone (H2A, H2B, H3 and H4) mRNAs were coordinately cell cycle-dependent and paralleled the rate of DNA synthesis during early germination, whereas the expression pattern of the linker histone (H1) genes differed. The five subclass histone genes were actively expressed in the meristematic tissues of young seedlings. Moreover, H1 genes were expressed in leaves that consist mostly of non-proliferating cells, in which core histone genes showed little expression. Quantitative alterations to the mRNAs of the putative transcription factors HBP-1a (17) and HBP-1b (c38) of wheat histone genes were similar to those of the core histone mRNAs, suggesting that both factors function in the cell cycle-dependent expression of wheat core histone genes.
在小麦种子早期萌发过程以及幼苗不同组织中，对编码五种组蛋白（H1、H2A、H2B、H3和H4）的基因以及假定的转录因子HBP-1a（17）和HBP-1b（c38）的表达情况进行了检测。核心组蛋白（H2A、H2B、H3和H4）mRNA的稳态水平在细胞周期中呈协同依赖性，并与早期萌发过程中的DNA合成速率平行，而连接组蛋白（H1）基因的表达模式则有所不同。这五个亚类组蛋白基因在幼苗的分生组织中活跃表达。此外，H1基因在主要由非增殖细胞组成的叶片中表达，而在这些叶片中核心组蛋白基因几乎不表达。小麦组蛋白基因假定转录因子HBP-1a（17）和HBP-1b（c38）的mRNA的定量变化与核心组蛋白mRNA的变化相似，这表明这两种因子在小麦核心组蛋白基因的细胞周期依赖性表达中发挥作用。
