Nasuda S, Liu Y G, Sakamoto A, Nakayama T, Iwabuchi M, Tsunewaki K
Laboratory of Genetics, Faculty of Agriculture, Kyoto University, Japan.
Plant Mol Biol. 1993 Jul;22(4):603-14. doi: 10.1007/BF00047401.
The chromosomal locations of the genes in common wheat that encode the five histones and five members of the HBP (histone gene-binding protein)-1 family were determined by hybridizing their cloned DNAs to genomic DNAs of nullitetrasomic and telosomic lines of common wheat, Triticum aestivum cv. Chinese Spring. The H1 and H2a genes are located on different sets of homoeologous chromosomes or chromosome arms, namely, 5A, 5B and 5D, and 2AS, 2BS and 2DS, respectively. Genes for the other histones, H2b, H3 and H4, are found in high copy number and are dispersed among a large number of chromosomes. The genes for all members of the HBP-1 family are present in small copy numbers. Those for HBP-1a(1) are located on six chromosome arms, 3BL, 5AL, 5DL, 6AL, 6BS and 7DL, whereas those for each HBP-1a(c14), 1a(17), 1b(c1), and 1b(c38) are on a single set of homoeologous chromosome arms; 4AS, 4BL, 4DL; 6AS, 6BS, 6DS; 3AL, 3BL, 3DL; and 3AS, 3BS, 3DS, respectively. The genes for histones H1 and H2a, and for all members of the HBP-1 family except HBP-1a(1) are assumed to have different phylogenetic origins. The genes for histone 2a and HBP-1a(17) are located in the RFLP maps of chromosomes 2B and 6A, respectively. Gene symbols are proposed for all genes whose chromosomal locations have been determined.
通过将编码5种组蛋白和HBP（组蛋白基因结合蛋白）-1家族5个成员的普通小麦基因的克隆DNA与普通小麦中国春品种的缺体四体和端体品系的基因组DNA杂交，确定了这些基因在染色体上的位置。H1和H2a基因分别位于不同的部分同源染色体或染色体臂上，即5A、5B和5D，以及2AS、2BS和2DS。其他组蛋白H2b、H3和H4的基因以高拷贝数存在，并分散在大量染色体中。HBP-1家族所有成员的基因拷贝数较少。HBP-1a(1)的基因位于6个染色体臂上，即3BL、5AL、5DL、6AL、6BS和7DL，而每个HBP-1a(c14)、1a(17)、1b(c1)和1b(c38)的基因分别位于一组部分同源染色体臂上；4AS、4BL、4DL；6AS、6BS、6DS；3AL、3BL、3DL；以及3AS、3BS、3DS。组蛋白H1和H2a以及除HBP-1a(1)之外的HBP-1家族所有成员的基因被认为具有不同的系统发育起源。组蛋白2a和HBP-1a(17)的基因分别位于染色体2B和6A的RFLP图谱中。为所有已确定染色体位置的基因提出了基因符号。
