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授粉和单性结实荔枝（荔枝 chinensis Sonn.）果实早期发育过程中的综合转录组学和蛋白质组学分析。

Comprehensive transcriptomics and proteomics analyses of pollinated and parthenocarpic litchi (Litchi chinensis Sonn.) fruits during early development.

机构信息

Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.

Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China.

出版信息

Sci Rep. 2017 Jul 14;7(1):5401. doi: 10.1038/s41598-017-05724-z.

DOI:10.1038/s41598-017-05724-z
PMID:28710486
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5511223/
Abstract

Litchi (Litchi chinensis Sonn.) is an important fruit that is widely cultivated in tropical and subtropical areas. In this study, we used RNA-Seq and iTRAQ technologies to compare the transcriptomes and proteomes of pollinated (polLFs) and parthenocarpic (parLFs) litchi fruits during early development (1 day, 2 days, 4 days and 6 days). We identified 4,864 DEGs in polLFs and 3,672 in parLFs, of which 2,835 were shared and 1,051 were specifically identified in parLFs. Compared to po1LFs, 768 DEGs were identified in parLFs. iTRAQ analysis identified 551 DEPs in polLFs and 1,021 in parLFs, of which 305 were shared and 526 were exclusively identified in parLFs. We found 1,127 DEPs in parLFs compared to polLFs at different stages. Further analysis revealed some DEGs/DEPs associated with abscisic acid, auxin, ethylene, gibberellin, heat shock protein (HSP), histone, ribosomal protein, transcription factor and zinc finger protein (ZFP). WGCNA identified a large set of co-expressed genes/proteins in polLFs and parLFs. In addition, a cross-comparison of transcriptomic and proteomic data identified 357 consistent DEGs/DEPs in polLFs and parLFs. This is the first time that protein/gene changes have been studied in polLFs and parLFs, and the findings improve our understanding of litchi parthenocarpy.

摘要

荔枝（Litchi chinensis Sonn.）是一种重要的水果，广泛种植于热带和亚热带地区。本研究采用 RNA-Seq 和 iTRAQ 技术，比较了授粉（polLFs）和单性结实（parLFs）荔枝果实早期发育（1 天、2 天、4 天和 6 天）过程中的转录组和蛋白质组。我们在 polLFs 中鉴定到 4864 个 DEGs，在 parLFs 中鉴定到 3672 个 DEGs，其中 2835 个在两者中共有，1051 个在 parLFs 中特有。与 polLFs 相比，parLFs 中鉴定到 768 个 DEGs。iTRAQ 分析在 polLFs 中鉴定到 551 个 DEPs，在 parLFs 中鉴定到 1021 个 DEPs，其中 305 个在两者中共有，526 个在 parLFs 中特有。与 polLFs 相比，parLFs 在不同发育阶段中鉴定到 1127 个 DEPs。进一步分析发现一些与脱落酸、生长素、乙烯、赤霉素、热休克蛋白（HSP）、组蛋白、核糖体蛋白、转录因子和锌指蛋白（ZFP）相关的 DEGs/DEPs。WGCNA 鉴定出大量在 polLFs 和 parLFs 中共表达的基因/蛋白。此外，转录组和蛋白质组数据的交叉比较鉴定到 357 个在 polLFs 和 parLFs 中一致的 DEGs/DEPs。这是首次研究 polLFs 和 parLFs 中的蛋白质/基因变化，研究结果提高了我们对荔枝单性结实的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/86749b941100/41598_2017_5724_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/e162296632b7/41598_2017_5724_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/085bd3ab7a45/41598_2017_5724_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/19aa9e771246/41598_2017_5724_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/a7a1bf48a917/41598_2017_5724_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/86749b941100/41598_2017_5724_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/e162296632b7/41598_2017_5724_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/085bd3ab7a45/41598_2017_5724_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/19aa9e771246/41598_2017_5724_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/a7a1bf48a917/41598_2017_5724_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db7b/5511223/86749b941100/41598_2017_5724_Fig5_HTML.jpg

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