Tabata T, Sasaki K, Iwabuchi M
Nucleic Acids Res. 1983 Sep 10;11(17):5865-75. doi: 10.1093/nar/11.17.5865.
Some wheat histone H4 genes have been cloned from a Charon 4 wheat genomic DNA library using sea urchin histone H4 DNA as a probe. DNA sequence analysis of a cloned gene showed that the deduced amino acid sequence of wheat histone H4 protein was identical to that of pea. The 5' end of wheat histone H4 mRNA was mapped on the cloned gene by the S1-procedure. Southern blotting analysis of the genomic DNA indicated that histone H4 genes were reiterated 100 to 125 times per hexaploid wheat genome.
利用海胆组蛋白H4 DNA作为探针,已从Charon 4小麦基因组DNA文库中克隆出一些小麦组蛋白H4基因。对一个克隆基因的DNA序列分析表明,小麦组蛋白H4蛋白的推导氨基酸序列与豌豆的相同。通过S1程序将小麦组蛋白H4 mRNA的5′末端定位在克隆基因上。基因组DNA的Southern印迹分析表明,组蛋白H4基因在六倍体小麦基因组中重复100至125次。