Tang K, Allman S L, Chen C H
Oak Ridge National Laboratory, Tennessee 37831.
Rapid Commun Mass Spectrom. 1993 Oct;7(10):943-8. doi: 10.1002/rcm.1290071016.
Ferulic acid, 3-hydroxypicolinic acid (3-HPA), and 2,5-dihydroxybenzoic acid (2,5-DHB) were used as matrices in MALDI of various oligonucleotides. It was found that 2,5-DHB is an excellent matrix for polydeoxyribothymidylic acid. A poly-T oligonucleotide with a size of 130 bases was successfully detected. 3-HPA was found to be a good matrix for MALDI of homo-oligomeric deoxynucleotides as well as those containing four different bases. Parent ions of pd(A)60, d(T)100, d(G)40, and d(C)40 were observed, and a mixed-base oligonucleotide of 150-mer was also detected. Polymer ions of poly-A as large as 420-mer were measured; however, the efficiency for detecting poly-T with 3-HPA as a matrix was slightly worse than when 2,5-DHB was used. Comparisons of three matrices for various oligonucleotides is also presented.
阿魏酸、3-羟基吡啶甲酸（3-HPA）和2,5-二羟基苯甲酸（2,5-DHB）被用作各种寡核苷酸基质辅助激光解吸电离（MALDI）的基质。研究发现，2,5-DHB是聚脱氧胸苷酸的优良基质。成功检测到了一条130个碱基的聚T寡核苷酸。结果表明，3-HPA是同聚寡聚脱氧核苷酸以及含有四种不同碱基的寡聚脱氧核苷酸MALDI分析的良好基质。观察到了pd(A)60、d(T)100、d(G)40和d(C)40的母离子，还检测到了一条150聚体的混合碱基寡核苷酸。测量了长达420聚体的聚A聚合物离子；然而，以3-HPA为基质检测聚T的效率略低于使用2,5-DHB时。还给出了三种基质对各种寡核苷酸的比较情况。
