寡脱氧核苷酸的红外和紫外基质辅助激光解吸/电离质谱分析比较
Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.
作者信息
Nordhoff E, Kirpekar F, Karas M, Cramer R, Hahner S, Hillenkamp F, Kristiansen K, Roepstroff P, Lezius A
机构信息
Institute for Medical Physics and Biophysics, Münster, Germany.
出版信息
Nucleic Acids Res. 1994 Jul 11;22(13):2460-5. doi: 10.1093/nar/22.13.2460.
Abstract
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
摘要
以3-羟基吡啶甲酸为基质的紫外基质辅助激光解吸/电离质谱（UV-MALDI-MS）和以琥珀酸为基质的红外基质辅助激光解吸/电离质谱（IR-MALDI-MS）已证明它们在核酸（DNA和RNA）高精度和高灵敏度质量测定方面的可行性。在这项工作中，对这两种基质辅助激光解吸/电离方法以及用于分析寡脱氧核苷酸的正离子和负离子质谱进行了详细比较。展示了多达40个核苷酸的DNA序列的质谱图。本研究中使用了线性和反射式飞行时间质谱分析仪，并比较了它们在寡脱氧核苷酸基质辅助激光解吸/电离分析中的潜力。还讨论了分子离子碎片化的作用。
相似文献
1
Comparison of IR- and UV-matrix-assisted laser desorption/ionization mass spectrometry of oligodeoxynucleotides.寡脱氧核苷酸的红外和紫外基质辅助激光解吸/电离质谱分析比较
Nucleic Acids Res. 1994 Jul 11;22(13):2460-5. doi: 10.1093/nar/22.13.2460.
2
Direct comparison of infrared and ultraviolet wavelength matrix-assisted laser desorption/ionization mass spectrometry of proteins.蛋白质的红外和紫外波长基质辅助激光解吸/电离质谱的直接比较。
J Am Soc Mass Spectrom. 1998 Jan;9(1):1-7. doi: 10.1016/S1044-0305(97)00236-5.
3
Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared.紫外和红外波长下核酸的基质辅助激光解吸/电离质谱分析
Rapid Commun Mass Spectrom. 1992 Dec;6(12):771-6. doi: 10.1002/rcm.1290061212.
4
Matrix-assisted laser desorption time-of-flight mass spectrometry of oligonucleotides using 3-hydroxypicolinic acid as an ultraviolet-sensitive matrix.使用3-羟基吡啶甲酸作为紫外线敏感基质的寡核苷酸基质辅助激光解吸飞行时间质谱分析。
Rapid Commun Mass Spectrom. 1993 Feb;7(2):142-6. doi: 10.1002/rcm.1290070206.
5
Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry.核酸质谱分析：基质辅助激光解吸电离（MALDI）质谱的前景。
Annu Rev Biophys Biomol Struct. 1995;24:117-40. doi: 10.1146/annurev.bb.24.060195.001001.
6
Ion stability of nucleic acids in infrared matrix-assisted laser desorption/ionization mass spectrometry.红外基质辅助激光解吸/电离质谱中核酸的离子稳定性
Nucleic Acids Res. 1993 Jul 25;21(15):3347-57. doi: 10.1093/nar/21.15.3347.
7
Picolinic acid as a matrix for laser mass spectrometry of nucleic acids and proteins.吡啶甲酸作为核酸和蛋白质激光质谱分析的基质。
Rapid Commun Mass Spectrom. 1994 Sep;8(9):673-7. doi: 10.1002/rcm.1290080902.
8
Matrix-assisted laser desorption/ionization time-of-flight analysis of Bacillus spores using a 2.94 microm infrared laser.使用2.94微米红外激光对芽孢杆菌孢子进行基质辅助激光解吸/电离飞行时间分析。
Rapid Commun Mass Spectrom. 2000;14(18):1701-6. doi: 10.1002/1097-0231(20000930)14:18<1701::AID-RCM82>3.0.CO;2-I.
9
Insight into absorption of radiation/energy transfer in infrared matrix-assisted laser desorption/ionization: the roles of matrices, water and metal substrates.深入了解红外基质辅助激光解吸/电离中的辐射吸收/能量转移：基质、水和金属基底的作用。
Rapid Commun Mass Spectrom. 1999;13(21):2191-8. doi: 10.1002/(SICI)1097-0231(19991115)13:21<2191::AID-RCM774>3.0.CO;2-I.
10
7-Deaza purine bases offer a higher ion stability in the analysis of DNA by matrix-assisted laser desorption/ionization mass spectrometry.在通过基质辅助激光解吸/电离质谱法分析DNA时，7-脱氮嘌呤碱基具有更高的离子稳定性。
Rapid Commun Mass Spectrom. 1995;9(6):525-31. doi: 10.1002/rcm.1290090611.
引用本文的文献
1
Synthesis and Investigation of Novel CHCA-Derived Matrices for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Lipids.新型 CHCA 衍生基质的合成与研究及其在脂质基质辅助激光解吸电离质谱分析中的应用。
Molecules. 2022 Apr 15;27(8):2565. doi: 10.3390/molecules27082565.
2
The development and application of matrix assisted laser desorption electrospray ionization: The teenage years.基质辅助激光解吸电喷雾电离的发展与应用：青春期。
Mass Spectrom Rev. 2023 Jan;42(1):35-66. doi: 10.1002/mas.21696. Epub 2021 May 24.
3
Production and analysis of multiply charged negative ions by liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry.采用液体常压基质辅助激光解吸/电离质谱法生成和分析多电荷负离子。
Rapid Commun Mass Spectrom. 2021 Jan;35 Suppl 1(Suppl 1):e8246. doi: 10.1002/rcm.8246. Epub 2018 Sep 12.
4
Mapping Post-Transcriptional Modifications onto Transfer Ribonucleic Acid Sequences by Liquid Chromatography Tandem Mass Spectrometry.通过液相色谱串联质谱法将转录后修饰映射到转运核糖核酸序列上
Biomolecules. 2017 Feb 22;7(1):21. doi: 10.3390/biom7010021.
5
Human Biomonitoring of DNA Adducts by Ion Trap Multistage Mass Spectrometry.利用离子阱多级质谱法对DNA加合物进行人体生物监测。
Curr Protoc Nucleic Acid Chem. 2016 Sep 1;66:7.24.1-7.24.25. doi: 10.1002/cpnc.12.
6
Mass spectrometry of structurally modified DNA.结构修饰DNA的质谱分析。
Chem Rev. 2013 Apr 10;113(4):2395-436. doi: 10.1021/cr300391r. Epub 2013 Feb 26.
7
Tandem mass spectrometry for characterization of covalent adducts of DNA with anticancer therapeutics.采用串联质谱法对与抗癌治疗药物发生共价结合的 DNA 进行特征分析。
Mass Spectrom Rev. 2013 Jul-Aug;32(4):247-66. doi: 10.1002/mas.21363. Epub 2012 Nov 13.
8
HPLC-UV, MALDI-TOF-MS and ESI-MS/MS analysis of the mechlorethamine DNA crosslink at a cytosine-cytosine mismatch pair.高效液相色谱-紫外、基质辅助激光解吸电离飞行时间质谱和电喷雾串联质谱分析在胞嘧啶-胞嘧啶错配碱基对处的氮芥 DNA 交联。
PLoS One. 2011;6(6):e20745. doi: 10.1371/journal.pone.0020745. Epub 2011 Jun 6.
9
Tag-mass: specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag.标签质谱法：基于光可裂解标签的质谱对转录组和蛋白质组的特异性分子成像
J Proteome Res. 2007 Jun;6(6):2057-67. doi: 10.1021/pr0700044. Epub 2007 May 4.
10
RNA fragmentation in MALDI mass spectrometry studied by H/D-exchange: mechanisms of general applicability to nucleic acids.通过氢/氘交换研究基质辅助激光解吸电离质谱中的RNA片段化：对核酸普遍适用的机制
J Am Soc Mass Spectrom. 2006 Oct;17(10):1353-1368. doi: 10.1016/j.jasms.2006.05.018. Epub 2006 Jul 27.
本文引用的文献
1
Antisense DNA oligonucleotides. II: The use of matrix-assisted laser desorption/ionization mass spectrometry for the sequence verification of methylphosphonate oligodeoxyribonucleotides.反义DNA寡核苷酸。II：基质辅助激光解吸/电离质谱法用于甲基膦酸寡脱氧核糖核苷酸序列验证
Rapid Commun Mass Spectrom. 1993 Mar;7(3):195-200. doi: 10.1002/rcm.1290070306.
2
Matrix-assisted laser desorption time-of-flight mass spectrometry of oligonucleotides using 3-hydroxypicolinic acid as an ultraviolet-sensitive matrix.使用3-羟基吡啶甲酸作为紫外线敏感基质的寡核苷酸基质辅助激光解吸飞行时间质谱分析。
Rapid Commun Mass Spectrom. 1993 Feb;7(2):142-6. doi: 10.1002/rcm.1290070206.
3
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a powerful tool for the mass and sequence analysis of natural and modified oligonucleotides.基质辅助激光解吸电离飞行时间质谱：用于天然和修饰寡核苷酸质量与序列分析的强大工具。
Nucleic Acids Res. 1993 Jul 11;21(14):3191-6. doi: 10.1093/nar/21.14.3191.
4
Basic matrices for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotides.用于蛋白质和寡核苷酸基质辅助激光解吸/电离质谱分析的基础基质。
Anal Chem. 1993 Nov 15;65(22):3204-11. doi: 10.1021/ac00070a007.
5
Matrix-assisted laser desorption/ionization Fourier-transform mass spectrometry of oligodeoxyribonucleotides.寡脱氧核糖核苷酸的基质辅助激光解吸/电离傅里叶变换质谱分析
Rapid Commun Mass Spectrom. 1993 Sep;7(9):828-36. doi: 10.1002/rcm.1290070910.
6
Matrix-assisted laser desorption ionization of oligonucleotides with various matrices.使用各种基质对寡核苷酸进行基质辅助激光解吸电离
Rapid Commun Mass Spectrom. 1993 Oct;7(10):943-8. doi: 10.1002/rcm.1290071016.
7
Ion stability of nucleic acids in infrared matrix-assisted laser desorption/ionization mass spectrometry.红外基质辅助激光解吸/电离质谱中核酸的离子稳定性
Nucleic Acids Res. 1993 Jul 25;21(15):3347-57. doi: 10.1093/nar/21.15.3347.
8
Ultrafast sequencing of oligodeoxyribonucleotides by FAB-mass spectrometry.用快原子轰击质谱法对寡脱氧核糖核苷酸进行超快测序
Nucleic Acids Res. 1982 Aug 11;10(15):4671-8. doi: 10.1093/nar/10.15.4671.
9
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
10
New developments in biochemical mass spectrometry: electrospray ionization.生化质谱的新进展：电喷雾电离
Anal Chem. 1990 May 1;62(9):882-99. doi: 10.1021/ac00208a002.
Zotero 插件