Nordhoff E, Kirpekar F, Karas M, Cramer R, Hahner S, Hillenkamp F, Kristiansen K, Roepstroff P, Lezius A
Institute for Medical Physics and Biophysics, Münster, Germany.
Nucleic Acids Res. 1994 Jul 11;22(13):2460-5. doi: 10.1093/nar/22.13.2460.
UV-matrix assisted laser desorption/ionization mass spectrometry (UV-MALDI-MS) with 3-hydroxypicolinic acid as matrix and IR-MALDI-MS with succinic acid as matrix have proved their feasibility for highly accurate and sensitive mass determination of nucleic acids (DNA and RNA). In this work, a detailed comparison of these two MALDI-methods and between positive- and negative ion mass spectra for the analysis of oligodeoxynucleotides is undertaken. Mass spectra of DNA sequences with up to 40 nucleotides are shown. Both linear and reflectron time-of-flight mass analyzers were used within this study and are compared for their potential in the MALDI analysis of oligodeoxynucleotides. The role of molecule-ion fragmentation is also discussed.
以3-羟基吡啶甲酸为基质的紫外基质辅助激光解吸/电离质谱（UV-MALDI-MS）和以琥珀酸为基质的红外基质辅助激光解吸/电离质谱（IR-MALDI-MS）已证明它们在核酸（DNA和RNA）高精度和高灵敏度质量测定方面的可行性。在这项工作中，对这两种基质辅助激光解吸/电离方法以及用于分析寡脱氧核苷酸的正离子和负离子质谱进行了详细比较。展示了多达40个核苷酸的DNA序列的质谱图。本研究中使用了线性和反射式飞行时间质谱分析仪，并比较了它们在寡脱氧核苷酸基质辅助激光解吸/电离分析中的潜力。还讨论了分子离子碎片化的作用。
