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基质辅助激光解吸/电离质谱法分析分子量高达150 kDa的酶促合成RNA。

Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa.

作者信息

Kirpekar F, Nordhoff E, Kristiansen K, Roepstorff P, Lezius A, Hahner S, Karas M, Hillenkamp F

机构信息

Department of Molecular Biology, Odense University, Denmark.

出版信息

Nucleic Acids Res. 1994 Sep 25;22(19):3866-70. doi: 10.1093/nar/22.19.3866.

DOI:10.1093/nar/22.19.3866
PMID:7524029
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308382/
Abstract

Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

摘要

通过基质辅助激光解吸/电离质谱（MALDI-MS）对酶促合成的RNA样本（体外转录本）进行分析。展示了分子量高达150 kDa（461个核苷酸）的RNA的光谱。讨论了聚合酶产生的样本异质性及其对质量分辨率的影响。对一个55 nt体外转录本的外切核酸酶消化时间进程进行分析，以研究MALDI-MS在复杂混合物上的性能。基于这些数据，讨论了通过MALDI-MS对由RNA聚合酶作用产生的DNA测序反应的分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0ea/308382/06353b6a95ca/nar00043-0064-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0ea/308382/f8b59eb8a25b/nar00043-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0ea/308382/e6bfd2482cfe/nar00043-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0ea/308382/06353b6a95ca/nar00043-0064-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0ea/308382/f8b59eb8a25b/nar00043-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0ea/308382/e6bfd2482cfe/nar00043-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0ea/308382/06353b6a95ca/nar00043-0064-b.jpg

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