Schlundt J, Munch B
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Zentralbl Bakteriol. 1993 Aug;279(3):336-43. doi: 10.1016/s0934-8840(11)80366-x.
A total of 481 samples of biomass from biogas plants treating slurry and other types of animal waste were examined for the presence of salmonellae by means of five different isolation methods. In 131 samples, Salmonella was isolated by means of one or more methods. A statistical evaluation of the isolation frequencies showed that Rappaport-Vassiliadis broth was significantly better than selenite broth with and without pre-enrichment and tetrathionate broth with pre-enrichment, whereas tetrathionate broth without pre-enrichment was significantly poorer than the other four methods. For each of the thirty different Salmonella serotypes, the isolation frequencies for the five methods are presented. Remarkably, Rappaport-Vassiliadis broth had very high isolation frequencies and tetrathionate broth with and without pre-enrichment had very low isolation frequencies for Salmonella typhimurium as well as Salmonella dublin.
采用五种不同的分离方法，对来自处理粪便和其他类型动物粪便的沼气厂的481份生物质样本进行了沙门氏菌检测。通过一种或多种方法，在131份样本中分离出了沙门氏菌。对分离频率的统计评估表明，Rappaport-Vassiliadis肉汤显著优于经或未经预富集的亚硒酸盐肉汤以及经预富集的四硫磺酸盐肉汤，而未经预富集的四硫磺酸盐肉汤则显著差于其他四种方法。针对30种不同的沙门氏菌血清型，分别列出了五种方法的分离频率。值得注意的是，对于鼠伤寒沙门氏菌和都柏林沙门氏菌，Rappaport-Vassiliadis肉汤的分离频率非常高，而经或未经预富集的四硫磺酸盐肉汤的分离频率则非常低。
