Hamid M E, Minnikin D E, Goodfellow M, Ridell M
Department of Chemistry, The University, Newcastle upon Tyne, U.K.
Zentralbl Bakteriol. 1993 Aug;279(3):354-67. doi: 10.1016/s0934-8840(11)80368-3.
Seventy-nine representative strains of Mycobacterium farcinogenes, Mycobacterium fortuitum, Mycobacterium peregrinum and Mycobacterium senegalense were analysed by thin-layer chromatography for diagnostic glycolipid and mycolic acid patterns. On the basis of glycolipid patterns the 16 M. senegalense strains were assigned to four groups (I, II, III and IV); one strain received as M. farcinogenes had glycolipids that corresponded to those given by the M. senegalense group III strain. Representatives of M. fortuitum produced alkalilabile glycolipids whereas the M. peregrinum strains produced alkali-stable glycopeptidolipids related to those of M. senegalense group I. Members of M. senegalense groups I, III and IV and the M. farcinogenes strains gave the characteristic pattern consisting of alpha- and epoxymycolates on thin-layer chromatographic analysis of the products of alkaline hydrolysis with 5% aqueous tetrabutylammonium hydroxide. Extraction of mycolates from the same strains using acid methanolysis revealed alpha-mycolates and characteristic more polar long-chain components originating from epoxymycolates. In contrast, the M. senegalense group II strains contained an additional more polar omega-1 methoxymycolate that was detected from both acid and alkaline hydrolysates; this spot was also seen in some M. fortuitum and M. peregrinum strains. Preliminary investigations, using a combination of thin-layer chromatography and immunostaining, showed that glycopeptidolipids from M. peregrinum and M. senegalense group I strains were antigenic, a cross-reaction between members of these taxa was shown. In contrast, no reaction was detected between glycopeptidolipids extracted from M. peregrinum and M. senegalense group I strains with antisera from either M. farcinogenes or M. fortuitum.
采用薄层色谱法对79株代表菌株——鼻疽分枝杆菌、偶然分枝杆菌、外源分枝杆菌和塞内加尔分枝杆菌进行分析，以确定其诊断性糖脂和分枝菌酸模式。根据糖脂模式，16株塞内加尔分枝杆菌菌株被分为四组（I、II、III和IV组）；一株被鉴定为鼻疽分枝杆菌的菌株具有与塞内加尔分枝杆菌III组菌株相同的糖脂。偶然分枝杆菌的代表菌株产生碱不稳定糖脂，而外源分枝杆菌菌株产生与塞内加尔分枝杆菌I组相关的碱稳定糖肽脂。塞内加尔分枝杆菌I、III和IV组的菌株以及鼻疽分枝杆菌菌株在用5%氢氧化四丁铵水溶液进行碱性水解产物的薄层色谱分析中呈现出由α-和环氧分枝菌酸组成的特征模式。使用酸性甲醇解从相同菌株中提取分枝菌酸，结果显示存在α-分枝菌酸以及源自环氧分枝菌酸的特征性极性更强的长链成分。相比之下，塞内加尔分枝杆菌II组菌株含有一种额外的极性更强的ω-1甲氧基分枝菌酸，在酸性和碱性水解产物中均能检测到；在一些偶然分枝杆菌和外源分枝杆菌菌株中也观察到了这个斑点。使用薄层色谱和免疫染色相结合的初步研究表明，外源分枝杆菌和塞内加尔分枝杆菌I组菌株的糖肽脂具有抗原性，这些分类群的成员之间存在交叉反应。相比之下，从外源分枝杆菌和塞内加尔分枝杆菌I组菌株中提取的糖肽脂与鼻疽分枝杆菌或偶然分枝杆菌的抗血清之间未检测到反应。
